Involvement of miR-21 in resistance to daunorubicin by regulating PTEN expression in the leukaemia K562 cell line

被引：88

作者：

Bai, Haitao ^{[1
]}

Xu, Rang ^{[2
]}

Cao, Zhongwei ^{[3
]}

Wei, Daolin ^{[1
]}

Wang, Chun ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Dept Haematol, Peoples Hosp 1, Shanghai 200030, Peoples R China

[2] Shanghai Jiao Tong Univ, Sci & Res Ctr, Affiliated Shanghai Xinhua Hosp, Shanghai 200030, Peoples R China

[3] Shanghai Jiao Tong Univ, Dept Gastroenterol, Affiliated Shanghai Peoples Hosp 1, Shanghai 200030, Peoples R China

来源：

FEBS LETTERS | 2011年 / 585卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Leukaemia; miR-21; Daunorubicin; Drug resistance; PTEN; PI3K/Akt; ACUTE MYELOID-LEUKEMIA; TUMOR-SUPPRESSOR GENE; PROSTATE-CANCER CELLS; ARSENIC TRIOXIDE; DRUG-RESISTANCE; TARGETING PTEN; BREAST-CANCER; IN-VIVO; MICRORNA-21; APOPTOSIS;

D O I：

10.1016/j.febslet.2010.12.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Recent studies have shown microRNA-21 (miR-21) is overexpressed in several types of cancer and contributes to tumor resistance to chemotherapy. In this study, we investigated whether miR-21 mediated resistance of the leukaemia cell line K562 to the chemotherapeutic agent daunorubicin (DNR). miR-21 expression was upregulated in the DNR resistant cell line K562/DNR compared to its parental line K562. Stable transfection of miR-21 induced drug resistance in K562, while suppression of miR-21 in K562/DNR led to enhanced DNR cytotoxicity. Additional experiments indicate that the mechanism of miR-21 drug resistance involves the PI3K/Akt pathway and changes following PTEN protein expression. This study provides a novel mechanism for understanding leukaemia drug resistance. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

收藏

页码：402 / 408

页数：7

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