Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine

被引：45

作者：

Hewavitharana, A. K. ^{[1
]}

Lee, S. ^{[2
]}

Dawson, P. A. ^{[2
]}

Markovich, D. ^{[2
]}

Shaw, P. N. ^{[1
]}

机构：

[1] Univ Queensland, Sch Pharm, Brisbane, Qld 4072, Australia

[2] Univ Queensland, Sch Biomed Sci, Brisbane, Qld 4072, Australia

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 374卷 / 01期

基金：

英国医学研究理事会; 澳大利亚研究理事会;

关键词：

HPLC; HPLC-MS; APAP; acetaminophen; metabolites; tandem MS;

D O I：

10.1016/j.ab.2007.11.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An HPLC-MS/MS method has been developed for the selective quantitative analysis of paracetamol and its two major metabolites. The use of tandem MS enabled the detection and quantitation of metabolites in small sample sizes with high sensitivity and selectivity. Isocratic elution using acetonitrile and water containing formic acid combined with electrospray-tandem MS enabled the separation and accurate quantitation of each analyte and the internal standard 3-acetamidophenol. The on-column limits of detection for paracetamol, paracetamol sulfate, and paracetamol glucuronide were 2.4, 1.2, and 1.2 pmol, respectively. The method was applied to quantitate paracetamol and its metabolites in mouse urine. It is highly specific, sensitive, and easily adaptable to measure these analytes in biological fluids of other animals. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：106 / 111

页数：6

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