Highly inclined thin illumination enables clear single-molecule imaging in cells

被引：892

作者：

Tokunaga, Makio ^{[1
,2
,3
]}

Imamoto, Naoko ^{[4
,5
]}

Sakata-Sogawa, Kumiko ^{[2
]}

机构：

[1] Natl Inst Genet, Biol Macromol Lab, Shizuoka 4118540, Japan

[2] RIKEN, Res Ctr Allergy & Immunol, Kanagawa 2300045, Japan

[3] Grad Univ Adv Studies, Sch Life Sci, Dept Genet, Shizuoka 4118540, Japan

[4] Natl Inst Genet, Gene Network Lab, Shizuoka 4118540, Japan

[5] RIKEN, Cellular Dynam Lab, Wako, Saitama 3510198, Japan

来源：

NATURE METHODS | 2008年 / 5卷 / 02期

关键词：

D O I：

10.1038/nmeth1171

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a simple illumination method of fluorescence microscopy for molecular imaging. Illumination by a highly inclined and thin beam increases image intensity and decreases background intensity, yielding a signal/background ratio about eightfold greater than that of epi-illumination. A high ratio yielded clear single-molecule images and three-dimensional images using cultured mammalian cells, enabling one to visualize and quantify molecular dynamics, interactions and kinetics in cells for molecular systems biology.

引用

页码：159 / 161

页数：3

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