Potentiation of humoral immune responses to vaccine antigens by recombinant chicken IL-18 (rChIL-18)

被引：63

作者：

Degen, WGJ ^{[1
]}

van Zuilekom, HI ^{[1
]}

Scholtes, NC ^{[1
]}

van Daal, N ^{[1
]}

Schijns, VEJC ^{[1
]}

机构：

[1] Intervet Int BV, Dept Vaccine Technol & Immunol R&D, NL-5830 AA Boxmeer, Netherlands

来源：

VACCINE | 2005年 / 23卷 / 33期

关键词：

chicken IL-1 8; adjuvant; antibody responses;

D O I：

10.1016/j.vaccine.2005.04.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mammalian interleukin-18 (IL-18) is one of the pro-inflammatory cytokines involved in innate immune responses to microbial infection preceding the induction of both cellular and humoral immune responses. We assessed the potential of Escherichia coli-expressed His-tag purified recombinant chicken IL-18 (rHis-ChIL-18) as a potentiator of vaccine-induced immune responses in 3 week-old SPF chickens and compared it with several commonly used traditional immunostimulating adjuvants. We found that rHis-ChIL-18 significantly enhanced antibody responses to Clostridium perfringens alpha-toxoid and Newcastle disease virus (NDV) antigens, comparable to the Al(OH)(3)-gel, Miglyol and chitosan adjuvant. However, antibody responses were lower than water-in-oil (w/o) emulsions and combinations of rHis-ChIL-18 with AI(OH)3-gel, Miglyol or chitosan. Improved HI-titers for infectious bronchitis virus (IBV) were not noted. We conclude that rHis-ChIL-18, when delivered in the proper context, is a novel and safe immunostimulator in chickens.

引用

页码：4212 / 4218

页数：7

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