Two-photon fluorescence correlation microscopy reveals the two-phase nature of transport in tumors

被引：141

作者：

Alexandrakis, G

Brown, EB

Tong, RT

McKee, TD

Campbell, RB

Boucher, Y

Jain, RK ^{[1
]}

机构：

[1] Massachusetts Gen Hosp, Dept Radiat Oncol, Edwin L Steele Lab, Boston, MA 02114 USA

[2] Harvard Univ, Sch Med, Boston, MA 02114 USA

[3] MIT, Biol Engn Div, Cambridge, MA 02139 USA

[4] Northeastern Univ, Bouve Coll Hlth Sci, Dept Pharmaceut Sci, Boston, MA 02115 USA

来源：

NATURE MEDICINE | 2004年 / 10卷 / 02期

关键词：

D O I：

10.1038/nm981

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transport parameters determine the access of drugs to tumors. However, technical difficulties preclude the measurement of these parameters deep inside living tissues. To this end, we adapted and further optimized two-photon fluorescence correlation microscopy (TPFCM) for in vivo measurement of transport parameters in tumors. TPFCM extends the detectable range of diffusion coefficients in tumors by one order of magnitude, and reveals both a fast and a slow component of diffusion. The ratio of these two components depends on molecular size and can be altered in vivo with hyaluronidase and collagenase. These studies indicate that TPFCM is a promising tool to dissect the barriers to drug delivery in tumors.

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收藏

页码：203 / 207

页数：5

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