A novel inhibitor protein for Bombyx cysteine proteinase is homologous to propeptide regions of cysteine proteinases

被引：27

作者：

Yamamoto, Y ^{[1
]}

Watabe, S

Kageyama, T

Takahashi, SY

机构：

[1] Yamaguchi Univ, Coll Agr, Dept Biochem & Radiat Biol, Yamaguchi 753, Japan

[2] Yamaguchi Univ, Radioisotopes Lab, Yamaguchi 753, Japan

[3] Kyoto Univ, Primate Res Inst, Dept Biochem, Inuyama, Aichi 484, Japan

来源：

FEBS LETTERS | 1999年 / 448卷 / 2-3期

关键词：

inhibitor; cysteine proteinase; propeptide region; cDNA cloning; sequence homology;

D O I：

10.1016/S0014-5793(99)00382-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone for an inhibitor of Bombyx cysteine proteinase was isolated and sequenced. Active inhibitor proteins were expressed in Escherichia coli using the cDNA. The open reading frame of the cDNA encodes a 105 residues protein with 19 residues of a signal sequence. The inhibitor has amino acid sequences homologous to several cysteine proteinases, but only to their propeptide sequences. The results suggest that some cysteine proteinase proregions may have evolved as autonomous modules and become inhibitor proteins for cysteine proteinases, (C) 1999 Federation of European Biochemical Societies.

引用

页码：257 / 260

页数：4

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