Electron transfer in human cytochrome P450 reductase

被引：24

作者：

Gutierrez, A

Grunau, A

Paine, M

Munro, AW

Wolf, CR

Roberts, GCK

Scrutton, NS

机构：

[1] Univ Leicester, Dept Biochem, Leicester LE1 7RH, Leics, England

[2] Univ Dundee, Ninewells Hosp & Med Sch, Ctr Biomed Res, Dundee DD1 9SY, Scotland

[3] Univ Leicester, Biol NMR Ctr, Leicester LE1 7RH, Leics, England

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2003年 / 31卷

关键词：

cytochrome P450 reductase; electron transfer; flavoprotein; kinetics;

D O I：

10.1042/bst0310497

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.

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收藏

页码：497 / 501

页数：5

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