Domain organization and functional analysis of Thermus thermophilus MutS protein

被引：17

作者：

Tachiki, H ^{[1
]}

Kato, R ^{[1
]}

Masui, R ^{[1
]}

Hasegawa, K ^{[1
]}

Itakura, H ^{[1
]}

Fukuyama, K ^{[1
]}

Kuramitsu, S ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Sci, Dept Biochem, Osaka 5600043, Japan

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 18期

关键词：

D O I：

10.1093/nar/26.18.4153

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MutS protein binds to DNA and specifically recognizes mismatched or small looped out heteroduplex DNA. In order to elucidate its structure-function relationships, the domain structure of Thermus thermophilus MutS protein was studied by performing denaturation experiments and limited proteolysis. The former suggested that T.thermophilus MutS consists of at least three domains with estimated stabilities of 12.3, 22.9 and 30.7 kcal/mol and the latter revealed that it consists of four domains: A1 (N-terminus to residue 130), A2 (131-274), B (275-570) and C (571 to C-terminus). A gel retardation assay indicated that T.thermophilus MutS interacts non-specifically with double-stranded (ds), but not single-stranded DNA. Among the proteolytic fragments, the B domain bound to dsDNA. On the basis of these results we have proposed the domain organization of T.thermophilus MutS and putative roles of these domains.

引用

页码：4153 / 4159

页数：7

共 28 条

[1] MutS mediates heteroduplex loop formation by a translocation mechanism [J].

Allen, DJ ;

Makhov, A ;

Grilley, M ;

Taylor, J ;

Thresher, R ;

Modrich, P ;

Griffith, JD .

EMBO JOURNAL, 1997, 16 (14) :4467-4476

[2]

CANFIELD RE, 1963, J BIOL CHEM, V238, P2698

[3] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].

DAVIS, BJ .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) :404-&

[4] THE MULTIPLICITY OF DOMAINS IN PROTEINS [J].

DOOLITTLE, RF .

ANNUAL REVIEW OF BIOCHEMISTRY, 1995, 64 :287-314

[5] MutS homologs in mammalian cells [J].

Fishel, R ;

Wilson, T .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1997, 7 (01) :105-113

[6]

FRIEDBERG EC, 1995, DNA REPAIR MUTAGENES

[7] The human mismatch recognition complex hMSH2-hMSH6 functions as a novel molecular switch [J].

Gradia, S ;

Acharya, S ;

Fishel, R .

CELL, 1997, 91 (07) :995-1005

[8] ALTERING THE CONSERVED NUCLEOTIDE BINDING MOTIF IN THE SALMONELLA-TYPHIMURIUM MUTS MISMATCH REPAIR PROTEIN AFFECTS BOTH ITS ATPASE AND MISMATCH BINDING ACTIVITIES [J].

HABER, LT ;

WALKER, GC .

EMBO JOURNAL, 1991, 10 (09) :2707-2715

[9] Cloning and characterization of the uvrD gene from an extremely thermophilic bacterium, Thermus thermophilus HB8 [J].

Hiramatsu, Y ;

Kato, R ;

Kawaguchi, S ;

Kuramitsu, S .

GENE, 1997, 199 (1-2) :77-82

[10] RECA PROTEIN FROM AN EXTREMELY THERMOPHILIC BACTERIUM, THERMUS-THERMOPHILUS HB8 [J].

KATO, R ;

KURAMITSU, S .

JOURNAL OF BIOCHEMISTRY, 1993, 114 (06) :926-929

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