Telomere-independent homologue pairing and checkpoint escape of accessory ring chromosomes in male mouse meiosis

被引:25
作者
Voet, T
Liebe, B
Labaere, C
Marynen, P
Scherthan, H [1 ]
机构
[1] Max Planck Inst Mol Genet, Dept Ropers, D-14195 Berlin, Germany
[2] Univ Leuven VIB, Dept Human Genet, Human Genome Lab, B-3000 Louvain, Belgium
关键词
chromosomal vector; spindle checkpoint; bouquet; synapsis; sex body;
D O I
10.1083/jcb.200305065
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We analyzed transmission of a ring minichromosome (MC) through mouse spermatogenesis as a monosome and in the presence of a homologue. Mice, either monosomic or disomic for the MC, produced MC+ offspring. In the monosomic condition, most univalents underwent self-synapsis as indicated by STAG3, SCP3, and SCP1 deposition. Fluorescent in situ hybridization and three-dimensional fluorescence microscopy revealed that ring MCs did not participate in meiotic telomere clustering while MC homologues paired at the XY-body periphery. Self-synapsis of MC(s) and association with the XY-body likely allowed them to pass putative pachytene checkpoints. At metaphase I and II, MC kinetochores assembled MAD2 and BUBR1 spindle checkpoint proteins. Unaligned MCs triggered the spindle checkpoint leading to apoptosis of metaphase cells. Other MCs frequently associated with mouse pericentric heterochromatin, which may have allowed them to pass the spindle checkpoint. Our findings indicate a telomere-independent mechanism for pairing of mammalian MCs, illuminate escape routes to meiotic checkpoints, and give clues for genetic engineering of germ line-permissive chromosomal vectors.
引用
收藏
页码:795 / 807
页数:13
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