Kinetic determinants of high-fidelity tRNA discrimination on the ribosome

被引：296

作者：

Gromadski, KB ^{[1
]}

Rodnina, MV ^{[1
]}

机构：

[1] Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

来源：

MOLECULAR CELL | 2004年 / 13卷 / 02期

关键词：

D O I：

10.1016/S1097-2765(04)00005-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ribosome selects aminoacyl-tRNA (aa-tRNA) matching to the mRNA codon from the bulk of nonmatching aa-tRNAs in two consecutive selection steps, initial selection and proofreading. Here we report the kinetic analysis of selection taking place under conditions where the overall selectivity was close to values observed in vivo and initial selection and proofreading contributed about equally. Comparison of the rate constants shows that the 350-fold difference in stabilities of cognate and near-cognate codon-anticodon complexes is not used for tRNA selection due to high rate of GTP hydrolysis in the cognate complex. tRNA selection at the initial selection step is entirely kinetically controlled and is due to much faster (650-fold) GTP hydrolysis of cognate compared to near-cognate substrate.

引用

页码：191 / 200

页数：10

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