Brain-derived neurotrophic factor (BDNF) is upregulated and associated with pain in chronic pancreatitis

被引:77
作者
Zhu, ZW
Friess, H [1 ]
Wang, L
Zimmermann, A
Büchler, MW
机构
[1] Univ Bern, Dept Visceral & Transplantat Surg, Inselspital, CH-3010 Bern, Switzerland
[2] Univ Bern, Inselspital, Inst Pathol, CH-3010 Bern, Switzerland
关键词
chronic pancreatitis; brain-derived neurotrophic factors; pain;
D O I
10.1023/A:1010684916863
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Our purpose was to investigate brain-derived neurotrophic factor (BDNF) in chronic pancreatitis (CP) in comparison with the normal pancreas and to evaluate its association with pain. By immunohistochemistry, in the normal pancreas BDNF immunoreactivity was moderately present in the cytoplasm of most ductal cells and weakly present in most acinar cells, islet cells, nerve fibers (including perineurium), and ganglia cells. In contrast, in CP intense immunostaining of BDNF was present in most cells of ductular complexes and in the perineurium of enlarged nerves. Moderate immunostaining of BDNF was found in degenerating acinar cells and islet cells. In addition, moderate immunoreactivity of BDNF was also detected in most enlarged nerve fibers and intrinsic pancreatic ganglia cells in CP samples. Western blot analysis also revealed 5.6-fold higher BDNF levels in CP samples (P < 0.01) compared with normal pancreas samples. The expression level of BDNF was positively correlated with pain intensity (P < 0.01) and pain frequency (P < 0.01) of CP patients. Furthermore, there was a significant relationship (r = 0.68, P < 0.01) between the BDNF immunostaining and the global pain scores. BDNF is increased in CP. Its association with pain suggests that it functions as a peripheral and central pain modulator, as reported previously in other inflammatory disorders.
引用
收藏
页码:1633 / 1639
页数:7
相关论文
共 25 条
[1]   Nerve growth factor regulates the expression of brain-derived neurotrophic factor mRNA in the peripheral nervous system [J].
Apfel, SC ;
Wright, DE ;
Wiideman, AM ;
Dormia, C ;
Snider, WD ;
Kessler, JA .
MOLECULAR AND CELLULAR NEUROSCIENCE, 1996, 7 (02) :134-142
[2]  
BUCHLER M, 1992, PANCREAS, V7, P183
[3]   Synaptic innervation density is regulated by neuron-derived BDNF [J].
Causing, CG ;
Gloster, A ;
Aloyz, R ;
Bamji, SX ;
Chang, E ;
Fawcett, J ;
Kuchel, G ;
Miller, FD .
NEURON, 1997, 18 (02) :257-267
[4]   Expression of mRNA for brain-derived neurotrophic factor in the dorsal root ganglion following peripheral inflammation [J].
Cho, HJ ;
Kim, SY ;
Park, MJ ;
Kim, DS ;
Kim, JK ;
Chu, MY .
BRAIN RESEARCH, 1997, 749 (02) :358-362
[5]   Increased brain-derived neurotrophic factor immunoreactivity in rat dorsal root ganglia and spinal cord following peripheral inflammation [J].
Cho, HJ ;
Kim, JK ;
Zhou, XF ;
Rush, RA .
BRAIN RESEARCH, 1997, 764 (1-2) :269-272
[6]  
Di Sebastiano P, 2000, Ann Ital Chir, V71, P11
[7]   Immune cell infiltration and growth-associated protein 43 expression correlate with pain in chronic pancreatitis [J].
DiSebastiano, P ;
Fink, T ;
Weihe, E ;
Friess, H ;
Innocenti, P ;
Beger, HG ;
Buchler, MW .
GASTROENTEROLOGY, 1997, 112 (05) :1648-1655
[8]   Neurotrophin signaling via Trks and p75 [J].
Friedman, WJ ;
Greene, LA .
EXPERIMENTAL CELL RESEARCH, 1999, 253 (01) :131-142
[9]   Nerve growth factor and its high-affinity receptor in chronic pancreatitis [J].
Friess, H ;
Zhu, ZW ;
di Mola, FF ;
Kulli, C ;
Graber, HU ;
Andren-Sandberg, Å ;
Zimmermann, A ;
Korc, M ;
Reinshagen, M ;
Büchler, MW .
ANNALS OF SURGERY, 1999, 230 (05) :615-624
[10]   Bilateral thoracoscopic splanchnicectomy:: Effects on pancreatic pain and function [J].
Ihse, I ;
Zoucas, E ;
Gyllstedt, E ;
Lillo-Gil, R ;
Andrén-Sandberg, Å .
ANNALS OF SURGERY, 1999, 230 (06) :785-790