The effect of 1-MCP on the expression of several ripening-related genes in strawberries

被引：18

作者：

Balogh, A ^{[1
]}

Koncz, T ^{[1
]}

Tisza, V ^{[1
]}

Kiss, E ^{[1
]}

Heszky, L ^{[1
]}

机构：

[1] St Istvan Univ, Dept Genet & Plant Breeding, H-2103 Godollo, Hungary

来源：

HORTSCIENCE | 2005年 / 40卷 / 07期

关键词：

cDNA-AFLP; cell wall; Fragaria x ananassa; gene expression;

D O I：

10.21273/HORTSCI.40.7.2088

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

To elucidate the role of ethylene in nonclimacteric fruit development and ripening, quantitative (cDNA-amplified fragment length polymorphism) cDNA-AFLP was used to visualize differential gene expression in four stages of ripening of strawberries (Fragaria xananassa Duch. 'Elsanta') treated with 1-methyleyclopropene (1-MCP), a competitive inhibitor of ethylene action. The proportion of clones affected by 1-MCP treatment was much higher in green than in white, pink, and red receptacle tissue. Three major cell-wall-related genes were affected by 1-MCP and, thus, are putatively ethylene dependent: a ripening-repressed beta-galactosidase (Fa beta gal3), up-regulated by 1-MCP; a putative endo-1,3-1,4-beta-D-glucanase (EGase), up-regulated in green and down-regulated in red fruit by 1-MCP; and a pectate lyase B (plB), expressed only in the red stage and significantly down-regulated by 1-MCP. Furthermore, we have identified genes encoding an alcohol dehydrogenase, a protein kinase-related protein, and a putative glutathione S-transferase, all ripening-induced and down-regulated by 1-MCP, suggesting that their regulation is at least partly ethylene dependent.

引用

页码：2088 / 2090

页数：3

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