Luminescence imaging microscopy and lifetime mapping using kinetically stable lanthanide(III) complexes

被引:209
作者
Beeby, A
Botchway, SW
Clarkson, IM
Faulkner, S
Parker, AW
Parker, D
Williams, JAG
机构
[1] Univ Durham, Dept Chem, Durham DH1 3LE, England
[2] Rutherford Appleton Lab, Lasers Sci Facil, Didcot OX11 0QX, Oxon, England
[3] Univ Surrey, Dept Chem, Guildford GU2 5XH, Surrey, England
关键词
lanthanide complexes; luminescence imaging microscopy; time-resolved images;
D O I
10.1016/S1011-1344(00)00070-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The sensitised luminescence from stable lanthanide complexes (1 and 2) bearing a phenanthridine antenna has been used to generate time-resolved images of silica particles. The millisecond order luminescent lifetime of these complexes is utilised to demonstrate time-gated imaging of the sample from a fluorescent background and to facilitate lifetime mapping over the area of the sample. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:83 / 89
页数:7
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