A Strategy for Direct Mapping and Identification of Mutations by Whole-Genome Sequencing

被引：117

作者：

Zuryn, Steven ^{[1
]}

Le Gras, Stephanie ^{[1
]}

Jamet, Karine ^{[1
]}

Jarriault, Sophie ^{[1
]}

机构：

[1] Univ Strasbourg, CNRS, UMR 1704, Inst Genet & Biol Mol & Cellulaire,INSERM,U964, F-67404 Strasbourg, France

来源：

GENETICS | 2010年 / 186卷 / 01期

关键词：

CAENORHABDITIS-ELEGANS; C-ELEGANS;

D O I：

10.1534/genetics.110.119230

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Mutant screens have proven powerful for genetic dissection of a myriad of biological processes, but subsequent identification and isolation of the causative mutations are usually complex and time consuming. We have made the process easier by establishing a novel strategy that employs whole-genome sequencing to simultaneously map and identify mutations without the need for any prior genetic mapping.

引用

页码：427 / 430

页数：4

共 24 条

[1] Sequence-based analysis of mutagenized mice [J].

Beier, DR .

MAMMALIAN GENOME, 2000, 11 (07) :594-597

[2] MAQGene: software to facilitate C. elegans mutant genome sequence analysis [J].

Bigelow, Henry ;

Doitsidou, Maria ;

Sarin, Sumeet ;

Hobert, Oliver .

NATURE METHODS, 2009, 6 (08) :549-549

[3] Identification of EMS-Induced Mutations in Drosophila melanogaster by Whole-Genome Sequencing [J].

Blumenstiel, Justin P. ;

Noll, Aaron C. ;

Griffiths, Jennifer A. ;

Perera, Anoja G. ;

Walton, Kendra N. ;

Gilliland, William D. ;

Hawley, R. Scott ;

Staehling-Hampton, Karen .

GENETICS, 2009, 182 (01) :25-32

[4]

BRENNER S, 1974, GENETICS, V77, P71

[5] Multiplex sequencing of plant chloroplast genomes using Solexa sequencing-by-synthesis technology [J].

Cronn, Richard ;

Liston, Aaron ;

Parks, Matthew ;

Gernandt, David S. ;

Shen, Rongkun ;

Mockler, Todd .

NUCLEIC ACIDS RESEARCH, 2008, 36 (19)

[6] Rapid single nucleotide polymorphism mapping in C-elegans -: art. no. 118 [J].

Davis, MW ;

Hammarlund, M ;

Harrach, T ;

Hullett, P ;

Olsen, S ;

Jorgensen, EM .

BMC GENOMICS, 2005, 6 (1)

[7] High mutation rate and predominance of insertions in the Caenorhabditis elegans nuclear genome [J].

Denver, DR ;

Morris, K ;

Lynch, M ;

Thomas, WK .

NATURE, 2004, 430 (7000) :679-682

[8] BIOCHEMISTRY OF MUTAGENESIS [J].

DRAKE, JW ;

BALTZ, RH .

ANNUAL REVIEW OF BIOCHEMISTRY, 1976, 45 :11-37

[9] Whole-genome sequencing and variant discovery in C-elegans [J].

Hillier, LaDeana W. ;

Marth, Gabor T. ;

Quinlan, Aaron R. ;

Dooling, David ;

Fewell, Ginger ;

Barnett, Derek ;

Fox, Paul ;

Glasscock, Jarret I. ;

Hickenbotham, Matthew ;

Huang, Weichun ;

Magrini, Vincent J. ;

Richt, Ryan J. ;

Sander, Sacha N. ;

Stewart, Donald A. ;

Stromberg, Michael ;

Tsung, Eric F. ;

Wylie, Todd ;

Schedl, Tim ;

Wilson, Richard K. ;

Mardis, Elaine R. .

NATURE METHODS, 2008, 5 (02) :183-188

[10] Mapping epigenetic mutations in fission yeast using whole-genome next-generation sequencing [J].

Irvine, Danielle V. ;

Goto, Derek B. ;

Vaughn, Matthew W. ;

Nakaseko, Yukinobu ;

McCombie, W. Richard ;

Yanagida, Mitsuhiro ;

Martienssen, Rob .

GENOME RESEARCH, 2009, 19 (06) :1077-1083

← 1 2 3 →