Identification of efficient fluorophores for the direct labeling of DNA via rolling circle amplification (RCA) polymerase φ29

被引：10

作者：

Linck, Lena ^{[1
,2
]}

Resch-Genger, Ute ^{[1
]}

机构：

[1] BAM Fed Inst Mat Res & Testing, D-12489 Berlin, Germany

[2] Free Univ Berlin, Inst Biochem, D-14195 Berlin, Germany

来源：

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY | 2010年 / 45卷 / 12期

关键词：

Rolling circle amplification (RCA); DNA; Fluorescent label; Fluorescence quantum yield; Cyanine; Stability; FLUORESCENT NUCLEOTIDES; QUANTUM DOTS; BODIPY; PROBES; DIMERS; DYES; DIAGNOSTICS; EMISSION; BASE; PCR;

D O I：

10.1016/j.ejmech.2010.09.005

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The enzymatic incorporation as well as the spectroscopic properties and photochemical stability of a series of fluorescent labels differing in dye class, charge, and rigidity were studied to identify new tools for signal enhancement in situ on microarrays without secondary labeling. These fluorophores were chosen to spectrally match or resemble the golden standard Cy3. With the rhodamine DY-555, that is three times more emissive than Cy3, we found a bright and stable chromophore, the spectroscopic properties of which are minimally influenced by dye microenvironment. (C) 2010 Elsevier Masson SAS. All rights reserved.

引用

页码：5561 / 5566

页数：6

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