Confirmatory real-time PCR assay for human papillomavirus (HPV) type 52 infection in anogenital specimens screened for HPV infection with the linear array HPV genotyping test

被引：45

作者：

Coutlee, Francois

Rouleau, Danielle

Ghattas, Georges

Hankins, Catherine

Vezina, Sylvie

Cote, Pierre

Macleod, John

de Pokomandy, Alexandra

Money, Deborah

Walmsley, Sharon

Voyer, Helene

Brassard, Paul

Franco, Eduardo

机构：

[1] Univ Montreal, Hop Notre Dame Ctr, Ctr Recherche, Dept Microbiol & Infectiol,Lab Virol Mol, Quebec City, PQ, Canada

[2] Univ Montreal, Hop Notre Dame Ctr, Ctr Recherche, Dept Family Med,Lab Virol Mol, Quebec City, PQ, Canada

[3] Univ Montreal, Hop Notre Dame Ctr, Ctr Recherche, Dept Gastro Enterol,Lab Virol Mol, Quebec City, PQ, Canada

[4] Univ Montreal, Dept Microbiol & Immunol, Quebec City, PQ, Canada

[5] McGill Univ, Dept Med, Div Canc Epedemiol, Quebec City, PQ, Canada

[6] Inst Natl Sante Publ Quebec, Direction Sante Publ Montreal Ctr, Montreal, PQ, Canada

[7] Clin Med Actuel, Quebec City, PQ, Canada

[8] Clin Med Quartier Latin, Montreal, PQ, Canada

[9] Univ British Columbia, Dept Obstet & Gynecol, Vancouver, BC, Canada

[10] Univ Toronto, Toronto Gen Hosp, Toronto Gen Res Inst, Div Clin Invest & Human Physiol, Toronto, ON, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2007年 / 45卷 / 11期

关键词：

D O I：

10.1128/JCM.01145-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel real-time PCR assay for detection of human papillomavirus type 52 (HPV-52) DNA (RT-52) was evaluated on 265 anogenital samples. RT-52 had a sensitivity of 98.4% and a specificity of 100% compared to conventional HPV-52 typing assays, including hybridization of PGMY products with an HPV-52-specific probe and PCR sequencing of HPV-52 E6.

引用

页码：3821 / 3823

页数：3

共 18 条

[1] Molecular analysis of human papillomavirus type 52 isolates detected in the genital tract of human immunodeficiency virus-seropositive and -seronegative women [J].

Aho, J ;

Hankins, C ;

Tremblay, C ;

Lang, F ;

Forest, P ;

Pourreaux, K ;

Rouah, F ;

Coutlée, F .

JOURNAL OF INFECTIOUS DISEASES, 2003, 188 (10) :1517-1527

[2] Human papillomavirus type 16 infections and 2-year absolute risk of cervical precancer in women with equivocal or mild cytologic abnormalities [J].

Castle, PE ;

Solomon, D ;

Schiffman, M ;

Wheeler, CM .

JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2005, 97 (14) :1066-1071

[3] Human papillomavirus genotype distribution in low-grade cervical lesions: Comparison by geographic region and with cervical cancer. [J].

Clifford, GM ;

Rana, RK ;

Franceschi, S ;

Smith, JS ;

Gough, G ;

Pimenta, JM .

CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, 2005, 14 (05) :1157-1164

[4] Human papillomavirus types in invasive cervical cancer worldwide:: a meta-analysis [J].

Clifford, GM ;

Smith, JS ;

Plummer, M ;

Muñoz, N ;

Franceschi, S .

BRITISH JOURNAL OF CANCER, 2003, 88 (01) :63-73

[5] Worldwide distribution of human papillomavirus types in cytologically normal women in the international Agency for Research on Cancer HPV prevalence surveys:: a pooled analysis [J].

Clifford, GM ;

Gallus, S ;

Herrero, R ;

Muñoz, N ;

Snijders, PJF ;

Vaccarella, S ;

Anh, PTH ;

Ferreccio, C ;

Hieu, NT ;

Matos, E ;

Molano, M ;

Rajkumar, R ;

Ronco, G ;

de Sanjosé, S ;

Shin, HR ;

Sukvirach, S ;

Thomas, JO ;

Tunsakul, S ;

Meijer, CJLM ;

Franceschi, S .

LANCET, 2005, 366 (9490) :991-998

[6] Use of PGMY primers in L1 consensus PCR improves detection of human papillomavirus DNA in genital samples [J].

Coutlée, F ;

Gravitt, P ;

Kornegay, J ;

Hankins, C ;

Richardson, H ;

Lapointe, N ;

Voyer, H ;

Franco, E .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (03) :902-907

[7]

Coutlée F, 1999, J CLIN MICROBIOL, V37, P1852

[8] Enhanced detection and typing of human papillomavirus (HPV) DNA in anogenital samples with PGMY primers and the linear array HPV genotyping test [J].

Coutlee, Francois ;

Rouleau, Danielle ;

Petignat, Patrick ;

Ghattas, Georges ;

Kornegay, Janet R. ;

Schlag, Peter ;

Boyle, Sean ;

Hankins, Catherine ;

Vezina, Sylvie ;

Cote, Pierre ;

Macleod, John ;

Voyer, Helene ;

Forest, Pierre ;

Walmsley, Sharon ;

Franco, Eduardo .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (06) :1998-2006

[9] Persistent human papillomavirus infection and cervical neoplasia [J].

Ferenczy, A ;

Franco, E .

LANCET ONCOLOGY, 2002, 3 (01) :11-16

[10]

Gravitt PE, 2000, J CLIN MICROBIOL, V38, P357

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