Role for toll-like receptor 4 in TNF-alpha secretion by murine macrophages in response to polysaccharide Krestin, a Trametes versicolor mushroom extract

被引：55

作者：

Price, Lisa A. ^{[1
]}

Wenner, Cynthia A. ^{[1
]}

Sloper, Daniel T. ^{[2
,3
]}

Slaton, Joel W. ^{[2
,3
]}

Novack, Jeffrey P. ^{[4
]}

机构：

[1] Bastyr Univ, Dept Basic Sci, Sch Nat Hlth Sci, Kenmore, WA 98028 USA

[2] Univ Minnesota, Dept Urol Surg, Minneapolis, MN 55455 USA

[3] Univ Minnesota, MN Vet Adm Med Ctr Minneapolis, Minneapolis, MN 55417 USA

[4] Bellevue Community Coll, Div Sci, Bellevue, WA 98007 USA

来源：

FITOTERAPIA | 2010年 / 81卷 / 07期

关键词：

Trametes versicolor; PSK; Dectin-1; receptor; Scleroglucan;

D O I：

10.1016/j.fitote.2010.06.002

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

Woody fungi and yeast preparations show promise in cancer treatment by activating antitumor immune responses. Macrophages (J774A.1) were treated with PSK, Reishi extract, scleroglucan or vehicle control. Pre-incubation with TLR4 blocking antibody inhibited TNF-alpha secretion by both J774A.1 cells and primary splenocytes but had inconclusive effect on scleroglucan-induced secretion of TNF-alpha. PSK induced TNF-alpha and IL-6 secretion by wild type but not by TLR4-deficient peritoneal macrophages. We conclude that constituents from PSK act as ligands for TLR4 receptors leading to induction of TNF-alpha and IL-6 inflammatory cytokines. Receptor-mediated differences may be due to structural differences in beta glucans or non-glucan constituents. (C) 2010 Published by Elsevier B.V.

引用

页码：914 / 919

页数：6

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