The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes

被引：43

作者：

Brems, S

Guilbride, DL

Gundlesdodjir-Planck, D

Busold, C

Luu, VD

Schanne, M

Hoheisel, J

Clayton, C

机构：

[1] ZMBH, D-69120 Heidelberg, Germany

[2] DKFZ, D-69120 Heidelberg, Germany

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2005年 / 139卷 / 02期

基金：

美国国家卫生研究院; 英国惠康基金;

关键词：

Trypanosoma; differentiation; transcript; regulation; array;

D O I：

10.1016/j.molbiopara.2004.11.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe developmentally regulated genes in two strains of Trypanosoma brucei: the monomorphic strain Lister 427 and the pleomorphic strain TREU927. Expression patterns were obtained using an array of 24,567 genomic fragments. Probes were prepared from bloodstream-form or procyclic-form trypanosomes. Fourteen procyclic-specific and 77 bloodstream-specific signals were obtained from sequences matching variant surface glycoprotein or associated genes, and a further 17 regulated sequences were repetitive or transposable-element-related. Two hundred and eighty-six regulated spots corresponded to mRNAs from other protein-coding genes; these spots represent 191 different proteins. Regulation of 113 different genes (79 from procyclic forms, 34 from bloodstream-forms) was supported by at least two independent experiments or criteria: of these, about 60 were novel. Only two genes - encoding HSP83 and an importin-related protein - appeared to be regulated in the TREU927 strain only. Our results confirmed previous estimates that 2% of trypanosome genes show developmental regulation at the mRNA level. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：163 / 172

页数：10

共 39 条

[1] Expression profiling using random genomic DNA microarrays identifies differentially expressed genes associated with three major developmental stages of the protozoan parasite Leishmania major [J].