PEGylated polyplex micelles from triblock catiomers with spatially ordered layering of condensed pDNA and buffering units for enhanced intracellular gene delivery

被引：183

作者：

Fukushima, S

Miyata, K

Nishiyama, N ^{[1
]}

Kanayama, N

Yamasaki, Y

Kataoka, K

机构：

[1] Univ Tokyo, Grad Sch Med, Ctr Dis Biol & Integrat Med, Tokyo 1130033, Japan

[2] Univ Tokyo, Grad Sch Engn, Dept Mat Sci & Engn, Bunkyo Ku, Tokyo 1138656, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 09期

关键词：

D O I：

10.1021/ja0440506

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An A-B-C type triblock copolymer, tandemly aligning two types of polycations with different pKa values in a single polymer strand, was developed for the construction of novel polyplex micelles, satisfying a high DNA condensing ability as well as a proton buffering activity directed to elevating gene transfection. The micelle might feature the distinctive three-layered structure, where an inner polyplex layer of condensed pDNA with poly(l-lysine) (pKa ∼9.4) as the C segment is successively wrapped with an intermediate layer of poly[(3-morpholinopropyl)aspartamide] (B segment) with a comparatively low pKa of∼6.2, to provide a buffering effect, and an outer PEG layer (A segment) as a biocompatible palisade. Copyright © 2005 American Chemical Society.

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页码：2810 / 2811

页数：2

相关论文

共 11 条

[1]

Behr JP, 1997, CHIMIA, V51, P34

[2] Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer [J].

Boeckle, S ;

von Gersdorff, K ;

van der Piepen, S ;

Culmsee, C ;

Wagner, E ;

Ogris, M .

JOURNAL OF GENE MEDICINE, 2004, 6 (10) :1102-1111

[3] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].

BOUSSIF, O ;

LEZOUALCH, F ;

ZANTA, MA ;

MERGNY, MD ;

SCHERMAN, D ;

DEMENEIX, B ;

BEHR, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7297-7301

[4] Effect of size and serum proteins on transfection efficiency of poly((2-dimethylamino)ethyl methacrylate)-plasmid nanoparticles [J].

Cherng, JY ;

vandeWetering, P ;

Talsma, H ;

Crommelin, DJA ;

Hennink, WE .

PHARMACEUTICAL RESEARCH, 1996, 13 (07) :1038-1042

[5] Polyion complex micelles as vectors in gene therapy - pharmacokinetics and in vivo gene transfer [J].

Harada-Shiba, M ;

Yamauchi, K ;

Harada, A ;

Takamisawa, I ;

Shimokado, K ;

Kataoka, K .

GENE THERAPY, 2002, 9 (06) :407-414

[6] Polyion complex micelles from plasmid DNA and poly(ethylene glycol)-poly(L-lysine) block copolymer as serum-tolerable polyplex system: physicochemical properties of micelles relevant to gene transfection efficiency [J].

Itaka, K ;

Yamauchi, K ;

Harada, A ;

Nakamura, K ;

Kawaguchi, H ;

Kataoka, K .

BIOMATERIALS, 2003, 24 (24) :4495-4506

[7] Evaluation by fluorescence resonance energy transfer of the stability of nonviral gene delivery vectors under physiological conditions [J].

Itaka, K ;

Harada, A ;

Nakamura, K ;

Kawaguchi, H ;

Kataoka, K .

BIOMACROMOLECULES, 2002, 3 (04) :841-845

[8] Soluble stoichiometric complexes from poly(N-ethyl-4-vinylpyridinium) cations and poly(ethylene oxide)-block-polymethacrylate anions [J].

Kabanov, AV ;

Bronich, TK ;

Kabanov, VA ;

Yu, K ;

Eisenberg, A .

MACROMOLECULES, 1996, 29 (21) :6797-6802

[9] Biodegradable, endosome disruptive, and cationic network-type polymer as a highly efficient and nontoxic gene delivery carrier [J].

Lim, YB ;

Kim, SM ;

Suh, H ;

Park, JS .

BIOCONJUGATE CHEMISTRY, 2002, 13 (05) :952-957

[10] Efficient gene transfer by histidylated polylysine pDNA complexes [J].

Midoux, P ;

Monsigny, M .

BIOCONJUGATE CHEMISTRY, 1999, 10 (03) :406-411