Varying susceptibility to myocardial infarction among C57BL/6 mice of different genetic background

被引：27

作者：

Gorog, DA ^{[1
]}

Tanno, M ^{[1
]}

Kabir, AMN ^{[1
]}

Kanaganayagam, GS ^{[1
]}

Bassi, R ^{[1
]}

Fisher, SG ^{[1
]}

Marber, MS ^{[1
]}

机构：

[1] St Thomas Hosp, Rayne Inst, Dept Cardiol, KCL, London SE1 7EH, England

来源：

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY | 2003年 / 35卷 / 06期

关键词：

knockout mice; myocardial infarction; congenic strains;

D O I：

10.1016/S0022-2828(03)00082-8

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Genetically manipulated mouse lines are invaluable to investigate the effects of a single gene on sensitivity to ischemia. When choosing appropriate controls, we were concerned that intrinsic, strain-independent but colony-dependent differences may influence the susceptibility to ischemia. We, therefore, compared the infarct:risk volume ratio (I:R%) after 30-min global ischemia in Langendorff-perfused hearts from outbred C57BL/6 mice with that in wild-type mice derived from heterozygote x heterozygote crosses of two different in-house C57BL/6 mouse lines with targeted disruption of an MKK3 or MAPKAPK2 allele. Despite similar hemodynamic characteristics, I:R% in outbred C57BL/6 hearts was significantly smaller (40.8 +/- 2.8%) than in C57BL/6 MAPKAPK2 wild types (65.8 +/- 4.5%, P = 0.0003) and significantly larger than in C57BL/6 MKK3 wild types (23.7 +/- 2.9%, P = 0.002). Therefore, inherent colony substrain-dependent differences appear to influence the susceptibility to infarction in response to global ischemia, underscoring the importance of using colony-matched wild-type controls in marine studies of myocardial ischemia. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：705 / 708

页数：4

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