Molecular cloning and characterization of a porcine UL16 binding protein (ULBP)-like cDNA

被引：13

作者：

García-Borges, CN

Phanavanh, B

Saraswati, S

Dennis, RA

Crew, MD

机构：

[1] Univ Arkansas Med Sci, Cent Arkansas Vet Healthcare Syst, Little Rock, AR 72205 USA

[2] Univ Arkansas Med Sci, Dept Biochem & Mol Biol, Little Rock, AR 72205 USA

[3] Univ Arkansas Med Sci, Dept Microbiol & Immunol, Little Rock, AR 72205 USA

[4] Univ Arkansas Med Sci, Dept Geriatr, Little Rock, AR 72205 USA

来源：

MOLECULAR IMMUNOLOGY | 2005年 / 42卷 / 06期

关键词：

NKG2D ligand; ULBP; pig; natural killer cells;

D O I：

10.1016/j.molimm.2004.09.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

UL16 binding proteins (ULBPs) are ligands for the NK cell activating receptor NKG2D. A cDNA encoding a porcine ULBP-like protein (PULBP) was cloned and the predicted amino acid sequence exhibited 35-52% identity to human ULBPs. Southern blot analysis suggested that there is only one ULBP-like gene in the pig genome. Transcripts of PULBP and another potential NKG2D ligand, MIC2, were detected by RT-PCR in a wide range of tissues. Recombinant PULBP-Fc and human ULBP2-Fc fusion proteins were made and used to examine PULBP binding to porcine PBMCs and a human NK cell line (NKL cells). PULBP-Fc bound to a subpopulation of porcine PBMCs but not NKL cells. Conversely. human ULBP2-Fc did not bind to porcine PBMCs but did stably interact with NKL cells. (c) 2004 Elsevier Ltd. All rights reserved.

引用

收藏

页码：665 / 671

页数：7

相关论文

共 25 条

[1] Isolation and characterization of immortalized porcine aortic endothelial cell lines [J].

Carrillo, A ;

Chamorro, S ;

Rodríguez-Gago, M ;

Alvarez, B ;

Molina, MJ ;

Rodríguez-Barbosa, JI ;

Sánchez, A ;

Ramírez, P ;

Muñoz, A ;

Domínguez, J ;

Parrilla, P ;

Yélamos, J .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2002, 89 (1-2) :91-98

[2] Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice [J].

Cerwenka, A ;

Bakker, ABH ;

McClanahan, T ;

Wagner, J ;

Wu, J ;

Phillips, JH ;

Lanier, LL .

IMMUNITY, 2000, 12 (06) :721-727

[3] Cutting edge:: The minor histocompatibility antigen H60 peptide interacts with both H-2Kb and NKG2D [J].

Cerwenka, A ;

O'Callaghan, CA ;

Hamerman, JA ;

Yadav, R ;

Ajayi, W ;

Roopenian, DC ;

Joyce, S ;

Lanier, LL .

JOURNAL OF IMMUNOLOGY, 2002, 168 (07) :3131-3134

[4] Sequence of the swine major histocompatibility complex region containing all non-classical class I genes [J].

Chardon, P ;

Rogel-Gaillard, C ;

Cattolico, L ;

Duprat, S ;

Vaiman, M ;

Renard, C .

TISSUE ANTIGENS, 2001, 57 (01) :55-65

[5]

Cosman D, 2001, IMMUNITY, V14, P123, DOI 10.1016/S1074-7613(01)00095-4

[6]

CREW MD, 1994, IMMUNOGENETICS, V39, P412, DOI 10.1007/BF00176158

[7] Rae1 and H60 ligands of the NKG2D receptor stimulate tumour immunity [J].

Diefenbach, A ;

Jensen, ER ;

Jamieson, AM ;

Raulet, DH .

NATURE, 2001, 413 (6852) :165-171

[8] Porcine gene discovery by normalized cDNA-library sequencing and EST cluster assembly [J].

Fahrenkrug, SC ;

Smith, TPL ;

Freking, BA ;

Cho, J ;

White, J ;

Vallet, J ;

Wise, T ;

Rohrer, G ;

Pertea, E ;

Sultana, R ;

Quackenbush, J ;

Keele, JW .

MAMMALIAN GENOME, 2002, 13 (08) :475-478

[9] Broad tumor-associated expression and recognition by tumor-derived γδ T cells of MICA and MICB [J].

Groh, V ;

Rhinehart, R ;

Secrist, H ;

Bauer, S ;

Grabstein, KH ;

Spies, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (12) :6879-6884

[10] Cell stress-regulated human major histocompatibility complex class I gene expressed in gastrointestinal epithelium [J].

Groh, V ;

Bahram, S ;

Bauer, S ;

Herman, A ;

Beauchamp, M ;

Spies, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (22) :12445-12450