On the structure of the scaffolding core of bacteriophage T4 and its role in head length determination

被引:7
作者
Berger, B
Shor, PW
机构
[1] MIT, Dept Math, Cambridge, MA 02139 USA
[2] MIT, Comp Sci Lab, Cambridge, MA 02139 USA
[3] AT&T Bell Labs, Florham Park, NJ 07932 USA
基金
美国国家科学基金会;
关键词
bacteriophage T4; scaffolding proteins; self-assembly; morphogenesis; Vernier mechanism;
D O I
10.1006/jsbi.1997.3942
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The scaffolding core in bacteriophages is a temporary structure that plays a major role in determining the shape of the protein shell that encapsulates the viral DNA. In the currently accepted structure for the scaffolding core in bacteriophage T4, there is a symmetry mismatch between the protein shell, which has fivefold symmetry, and the scaffolding core, which is believed to consist of six helical chains. Alternate structures for the scaffolding core in T4 are investigated. Starting with the hypothesis that the shell and a 10-helix core would have matching symmetry, a Vernier mechanism is proposed that explains the previously unexplained behavior of the length determination process in giant head mutants of T4. Other possible Vernier mechanisms for core structures containing six and eight helices are also explored. (C) 1998 Academic Press.
引用
收藏
页码:285 / 294
页数:10
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