A rapid method for the separation and analysis of carrageenan oligosaccharides released by iota- and kappa-carrageenase

被引：43

作者：

Knutsen, SH

Sletmoen, M

Kristensen, T

Barbeyron, T

Kloareg, B

Potin, P

机构：

[1] Agr Univ Norway, Inst Chem & Biotechnol, N-1432 As, Norway

[2] Biol Stn, UMR 1931 CNRS Goemar, F-29682 Roscoff, France

来源：

CARBOHYDRATE RESEARCH | 2001年 / 331卷 / 01期

关键词：

carrageenan; Iota-carrageenase; Kappa-carrageenase; sulphated neocarrabiose oligosaccharides; size-exclusion chromatography; NMR;

D O I：

10.1016/S0008-6215(00)00324-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Based on the improved performances in speed of chromatographic separation on Superdex-type materials (Pharmacia) compared to conventional media such as Sephadex and Bio Gel-type, a rapid size-exclusion chromatography (SEC) method was developed for the separation and analysis of carrageenan oligosaccharides. It was used to evaluate the elution profiles of hydrolysates produced by carrageenases specific for kappa- and iota-carrageenans. Oligosaccharide peaks ranging from di- to dodeca-saccharides were obtained in about 20 min on an analytical scale, whereas preparative runs were completed in a few hours. The method may also be used to monitor polysaccharide degradation. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：101 / 106

页数：6

共 20 条

[1] ι-Carrageenases constitute a novel family of glycoside hydrolases, unrelated to that of κ-carrageenases [J].