A candidate gene for Drosophila genome methylation

被引：14

作者：

Reddy, MN ^{[1
]}

Tang, LY ^{[1
]}

Lee, TL ^{[1
]}

Shen, CKJ ^{[1
]}

机构：

[1] Acad Sinica, Inst Mol Biol, Taipei 115, Taiwan

来源：

ONCOGENE | 2003年 / 22卷 / 40期

关键词：

DNA methylation; Drosophila cells; enzyme;

D O I：

10.1038/sj.onc.1206650

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Like vertebrates, the genome of Drosophila melanogaster also contains methylated cytosines. However, the enzyme( s) responsible for this methylation has been elusive. By DNA transfection and sodium bisulfite sequencing, we show here that overexpression of dDnmt2, which is the only expressed and cloned Drosophila protein consisting of motifs conserved among the DNA cytosine methyltransferases, results in genomic DNA methylation of Drosophila S2 cells. The data provide the first evidence for dDnmt2 being one candidate gene encoding the Drosophila DNA methyltransferase(s).

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收藏

页码：6301 / 6303

页数：3

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