Biological and molecular characterization of PNH-like lymphocytes emerging after Campath-1H therapy

被引：14

作者：

Fracchiolla, NS ^{[1
]}

Barcellini, W ^{[1
]}

Bianchi, P ^{[1
]}

Motta, M ^{[1
]}

Fermo, E ^{[1
]}

Cortelezzi, A ^{[1
]}

机构：

[1] Osped Maggiore Policlin IRCCS, Dipartimento Ematol, I-20122 Milan, Italy

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2001年 / 112卷 / 04期

关键词：

Campath-1H; CLL; cytokines; PIG-A; PNH-like lymphocytes;

D O I：

10.1046/j.1365-2141.2001.02677.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Campath-1H, an anti-CD52 monoclonal antibody, is therapeutically active in lymphoproliferative and autoimmune diseases. After Campath-1H therapy, lymphocytes with a paroxysmal nocturnal haemoglobinuria (PNH) phenotype have been reported to emerge. We characterized a PNH-like lymphocyte population emerging after Campath-1H therapy, in a patient with fludarabine refractory B-cell chronic lymphocytic leukaemia (B-CLL). We demonstrated a reduction in PIG-A mRNA levels compared with controls, and of all cytokines tested [interleukin (IL)-4, IL-13, IL-2, interferon(IFN)-gamma, IL-6, IL-10, and tumour necrosis factor (TNF)-alpha], except transforming growth factor (TGF)-beta, Given the inhibitory activity of TGF-beta, its elevated levels may contribute to the selective pressure of Campath-1H, leading to the emergence of PNH-like lymphocytes.

引用

页码：969 / 971

页数：3

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