H+/e(-) stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells

被引：68

作者：

Bogachev, AV ^{[1
]}

Murtazina, RA ^{[1
]}

Skulachev, VP ^{[1
]}

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV,AN BELOZERSKY INST PHYSICOCHEM BIOL,DEPT BIOENERGET,MOSCOW 119899,RUSSIA

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 21期

关键词：

D O I：

10.1128/jb.178.21.6233-6237.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Anaerobically grown Escherichia coli cells were shown to acidify the reaction medium in response to oxygen or dimethyl sulfoxide (DMSO) pulses, with the H+/e(-) stoichiometry being close to 2.5 and 1.5, respectively. In the presence of the NADH dehydrogenase I (NDH-I) inhibitor 8-methyl-N-vanillyl-6-nonenamide (capsaicin) or in mutants lacking NDH-I, this ratio decreased to 1 for O-2 and to 0 for DMSO. These data suggest that (i) the H+/e(-) stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force.

引用

页码：6233 / 6237

页数：5

共 32 条

[1]

BILOUS PT, 1985, J BACTERIOL, V163, P369, DOI 10.1128/JB.163.1.369-375.1985

[2]

NUCLEOTIDE-SEQUENCE OF THE DMSABC OPERON ENCODING THE ANAEROBIC DIMETHYLSULFOXIDE REDUCTASE OF ESCHERICHIA-COLI [J].