Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene

被引：31

作者：

Bahl, MI ^{[1
]}

Hansen, LH ^{[1
]}

Sorensen, SJ ^{[1
]}

机构：

[1] Univ Copenhagen, Dept Microbiol, DK-1307 Copenhagen, Denmark

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 253卷 / 02期

关键词：

biosensor; flow cytometry; tetracycline;

D O I：

10.1016/j.femsle.2005.09.034

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose-response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ng ml(-1) to 16 mu g ml(-1) which represents a significant improvement of the original version. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：201 / 205

页数：5

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