Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales

被引:43
作者
Azuma, KyoIchi
Kobayashi, Masaki
Nakamura, Masahisa [1 ]
Suzuki, Nobuo
Yashima, Sayaka
Iwamuro, Shawichi
Ikegame, Mika
Yamamoto, Toshio
Hattori, Atsuhiko
机构
[1] Waseda Univ, Fac Educ & Integrated Arts & Sci, Dept Biol, Tokyo 1698050, Japan
[2] Kanazawa Univ, Inst Nat & Environm Technol, Noto Marine Lab, Ishikawa 9270553, Japan
[3] Toho Univ, Fac Sci, Dept Biol, Chiba 2748510, Japan
[4] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Oral Morphol, Okayama 7008525, Japan
[5] Tokyo Med & Dent Univ, Coll Liberal Arts & Sci, Dept Biol, Chiba 2720827, Japan
基金
日本学术振兴会;
关键词
tartrate-resistant acid phosphatase; cathepsin K; sequence; scale; bone; osteoclasts; morphology; goldfish;
D O I
10.1016/j.bbrc.2007.08.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
Complementary DNAs encoding two major osteoclastic markers, tartrate-resistant acid phosphatase (TRAP) and cathepsin K (Cath K) were cloned from the scales of a teleost, the goldfish. This is the first report of the full coding sequence of TRAP and Cath K molecules in fish. In the goldfish scale both TRAP and Cath K mRNAs were expressed in the multinucleate osteoclasts, which showed large numbers of mitochondria and lysosomes, and a well developed ruffled border. These characteristic features of osteoclasts in the scales are similar to those in mammals. Most teleosts use the scale as an internal calcium reservoir during the reproductive season. The expression of TRAP and Cath K mRNAs in the scale significantly increased in April, which is a reproductive season, compared with that in October, a non-reproductive season. Thus, both of these molecular markers should be useful for the study of osteoclasts in the teleost scale. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:594 / 600
页数:7
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