Effect of mutations in Pisum sativum L. genes blocking different stages of nodule development on the expression of late symbiotic genes in Rhizobium leguminosarum bv. viciae

被引:31
作者
Voroshilova, VA
Boesten, B
Tsyganov, VE
Borisov, AY
Tikhonovich, IA
Priefer, UB
机构
[1] All Russia Res Inst Agr Microbiol, St Petersburg 196608 8, Pushkin, Russia
[2] Rhein Westfal TH Aachen, D-52056 Aachen, Germany
关键词
D O I
10.1094/MPMI.2001.14.4.471
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this report, the expression of late symbiotic genes (fnrN, fixN, and nifA) of Rhizobium leguminosarum by. viciae was studied in nodules of mutant pea lines blocked at four successive stages of nodule development. Bacterial gene expression was analyzed in situ with transcriptional gusA reporter gene fusions. As a control, a constitutively expressed gusA gene was included. In the nodules of Nop(-) (nodule persistence) mutants (mutant in gene sym13), which had not yet exhibited signs of premature senescence, the expression patterns observed were identical to those in wild-type nodules. Normal expression of fusions also occurred in nodules defective at the infection droplet differentiation stage (mutant in gene sym40) in which bacteria are endocytosed, but infection threads and infection droplets are hypertrophied. In contrast, in Itn(-) (infection thread formation inside the nodule tissue) mutants (mutant gene sym33), in which there is no endocytosis of bacteria, expression of the constitutive fusion was only in infection threads and no activity was shown for the other fusions. From this it can be concluded that functionality of the plant gene Sym33, i.e., bacterial endocytosis, is a prerequisite for the expression of late symbiotic genes in the microsymbiont. No morphologically distinct interzone II-III could be detected in nodules blocked at the bacteroid differentiation stage (mutants in gene sym31). The constitutive fusion was expressed equally throughout the nodule tissue (except for the meristem), and the activity of fusions to late symbiotic genes increased gradually with a maximal expression level at the base of the nodule. This is consistent with an altered oxygen barrier previously reported for these nodules. By including double mutants, earlier results on sequential functioning of gene pairs sym33-sym40 and sym31-sym13 could be confirmed and it could be demonstrated that the developmental epistasis found at the morphological level also is reflected in the expression pattern of late symbiotic genes in the microsymbiont.
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页码:471 / 476
页数:6
相关论文
共 52 条
[1]   TE7, AN INEFFICIENT SYMBIOTIC MUTANT OF MEDICAGO-TRUNCATULA GAERTN CV JEMALONG [J].
BENABEN, V ;
DUC, G ;
LEFEBVRE, V ;
HUGUET, T .
PLANT PHYSIOLOGY, 1995, 107 (01) :53-62
[2]  
BERDNIKOV VA, 1989, C GEN MAY 23 25 KIEV, V2, P26
[3]  
BERINGER JE, 1974, J GEN MICROBIOL, V84, P188
[4]  
Borisov A. Y., 1992, Symbiosis (Rehovot), V14, P297
[5]  
Borisov A. Y., 1999, Pisum Genetics, V31, P40
[6]   Sequential functioning of Sym-13 and Sym-31, two genes affecting symbiosome development in root nodules of pea (Pisum sativum L) [J].
Borisov, AY ;
Rozov, SM ;
Tsyganov, VE ;
Morzhina, EV ;
Lebsky, VK ;
Tikhonovich, IA .
MOLECULAR AND GENERAL GENETICS, 1997, 254 (05) :592-598
[7]  
BORISOV AY, 1997, NITROGEN FIXATION 21
[8]   PLANT GENETIC-CONTROL OF NODULATION [J].
CAETANOANOLLES, G ;
GRESSHOFF, PM .
ANNUAL REVIEW OF MICROBIOLOGY, 1991, 45 :345-382
[9]   AN FNR-LIKE PROTEIN ENCODED IN RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE SHOWS STRUCTURAL AND FUNCTIONAL HOMOLOGY TO RHIZOBIUM-MELILOTI FIXK [J].
COLONNAROMANO, S ;
ARNOLD, W ;
SCHLUTER, A ;
BOISTARD, P ;
PUHLER, A ;
PRIEFER, UB .
MOLECULAR & GENERAL GENETICS, 1990, 223 (01) :138-147
[10]   Host genetic control of symbiosis in soybean (Glycine max L) [J].
Devine, TE ;
Kuykendall, LD .
PLANT AND SOIL, 1996, 186 (01) :173-187