A specific binding site for neoxanthin in the monomeric antenna proteins CP26 and CP29 of Photosystem II

被引：66

作者：

Caffarri, Stefano

Passarini, Francesca

Bassi, Roberto

Croce, Roberta

机构：

[1] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Biophys Chem, NL-9747 AG Groningen, Netherlands

[2] Univ Aix Marseille 2, CNRS, CEA, UMR 6191,Lab Genet Biophys Plante, F-13288 Marseille, France

[3] Univ Verona, Dipartimento Sci Tecnol, I-37134 Verona, Italy

来源：

FEBS LETTERS | 2007年 / 581卷 / 24期

关键词：

photosynthesis; CP29; CP26; neoxanthin; light-harvesting complex; Photosystem II;

D O I：

10.1016/j.febslet.2007.08.066

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The location of the neoxanthin binding site in CP26 and CP29 was investigated by site-directed mutagenesis. The crystallographic structure of LHCII shows that the binding of neoxanthin to the NI site is stabilised by an H bond with a tyrosine in the lumenal loop. This residue is conserved in CP26 and CP29. Mutation of this tyrosine into phenylalanine induced specific loss of neoxanthin without affecting violaxanthin binding. In contrast to previous proposals, it is thus concluded that also in these minor antenna complexes neoxanthin is accommodated in the NI site. The characteristics of this binding site in the different antenna complexes are discussed. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4704 / 4710

页数：7

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