Overexpression of glutamate decarboxylase in transgenic tobacco plants deters feeding by phytophagous insect larvae

被引:62
作者
MacGregor, KB
Shelp, BJ
Peiris, S
Bown, AW [1 ]
机构
[1] Brock Univ, Dept Biol Sci, St Catharines, ON L2S 3A1, Canada
[2] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
gamma-aminobutyrate; GABA; glutamate decarboxylase; phytophagous; plant resistance; insect larvae; transgenic plants; invertebrate pests;
D O I
10.1023/A:1025650914947
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gamma-aminobutyrate (GABA) is a ubiquitous four-carbon, nonprotein amino acid synthesized by glutamate decarboxylase. Previous research suggests that the endogenous synthesis of GABA, a naturally occurring inhibitory neurotransmitter at neuromuscular junctions, serves as a plant resistance mechanism against invertebrate pests. In this study, two homozygous transgenic tobacco lines constitutively overexpressing a single copy of a full-length chimeric glutamate decarboxylase cDNA and possessing enhanced capacity for GABA accumulation (GAD plants), a homozygous transgenic line lacking the gene insert, and wild-type tobacco were employed. Tobacco budworm larvae were presented with plantattached wild type and transgenic leaves for 4 hr in a feeding preference study. Larvae consumed six to twelve times more leaf tissue from wild-type plants than from GAD plants. These results suggest that leaf GABA accumulation, which is known to occur in response to insect larval walking and feeding, represents a rapidly deployed localresistance mechanism.
引用
收藏
页码:2177 / 2182
页数:6
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