Cytotoxic T lymphocyte reactivity to gp100, MelanA/MART-1, and tyrosinase, in HLA-A2-positive vitiligo patients

被引:82
作者
Mandelcorn-Monson, RL
Shear, NH
Yau, E
Sambhara, S
Barber, BH
Spaner, D
DeBenedette, MA
机构
[1] Aventis Pasteur Canada Ltd, Res Ctr, Immunol Platform, N York, ON M2R 3T4, Canada
[2] Univ Toronto, Dept Med, Sunnybrook & Womens Coll Hlth Sci Ctr, Div Dermatol, Toronto, ON, Canada
[3] Univ Toronto, Dept Med, Sunnybrook & Womens Coll Hlth Sci Ctr, Res Inst,Div Mol & Cellular Biol, Toronto, ON, Canada
关键词
CD8(+) T cells; enzyme-linked immunospot assay; melanocyte antigens; melanoma; pigmentary disorders;
D O I
10.1046/j.1523-1747.2003.12413.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Vitiligo is a common depigmentation disorder thought to result from autoimmune destruction of melanocytes. Recent studies suggest a role for cell-mediated immune responses to melanocyte differentiation antigens, including gp100, MelanA/MART-1, and tyrosinase, in vitiligo pathogenesis. This study investigated T cell reactivity to MelanA/MART-1, tyrosinase, and gp100, in HLA-A2-positive patients with vitiligo. Melanocyte-specific T cell responses were measured ex vivo via enzyme-linked immunospot assay following stimulation with MelanA/MART-1, tyrosinase, and modified gp100 epitopes. Antigen-specific T lymphocyte reactivity to gp100 peptides was seen in 15 of 17 (88%) patients, with many demonstrating very high reactivity at levels comparable with those observed with common recall antigens. Reactivity to gp100 was noted to be associated with disease activity. Antigen-specific T lymphocyte reactivity to MelanA/MART-1 and tyrosinase peptides was not observed ex vivo in our patients, and only one patient demonstrated responses to MelanA/MART-1 and tyrosinase peptides following in vitro re-stimulation. Our findings implicate T cell reactivity to gp100 in patients with active disease and support the concept of an immunopathologic mechanism in vitiligo, in which cell-mediated responses to normal melanocyte antigens play a crucial part.
引用
收藏
页码:550 / 556
页数:7
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