Label-Free Indirect Immunoassay Using an Avidin-Induced Surface-Enhanced Raman Scattering Substrate

被引：34

作者：

Han, Xiao Xia ^{[1
,2
]}

Chen, Lei ^{[3
]}

Ji, Wei ^{[3
]}

Xie, Yunfei ^{[3
]}

Zhao, Bing ^{[3
]}

Ozaki, Yukihiro ^{[1
,2
]}

机构：

[1] Kwansei Gakuin Univ, Sch Sci & Technol, Dept Chem, Sanda, Hyogo 6691337, Japan

[2] Kwansei Gakuin Univ, Sch Sci & Technol, Res Ctr Single Mol Vibrat Spect, Sanda, Hyogo 6691337, Japan

[3] Jilin Univ, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China

来源：

SMALL | 2011年 / 7卷 / 03期

关键词：

PREPARED SILVER SURFACE; SPECTROSCOPY; ANTIGEN; ADSORPTION; SERS; RECOGNITION; MECHANISMS; PEPTIDES; PROTEINS; BINDING;

D O I：

10.1002/smll.201001936

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A label-free immunoassay based on a simple, surface-enhanced Raman scattering (SERS) approach is reported. Biotin-conjugated antibodies are attached to avidin-aggregated Ag nanoparticles via biotin-avidin biorecognition. More highly sensitive protein detection and more biocompatible protein adsorption are achieved compared to previous relevant methods. Importantly, a SERS intensity ratio of two bands is found to be sensitive to the concentration of corresponding antigens. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：316 / 320

页数：5

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