Structure, function, and, motility of vacuoles in filamentous fungi

被引:92
作者
Cole, L [1 ]
Orlovich, DA [1 ]
Ashford, AE [1 ]
机构
[1] Univ New S Wales, Sch Biol Sci, Sydney, NSW 2052, Australia
基金
澳大利亚研究理事会;
关键词
endocytosis; fluorescent probes; FM4-64; freeze substitution; fungal transport; MDY-64; motile tubular vacuole; Oregon green 488; phosphorus storage; Pisolithus tinctorius;
D O I
10.1006/fgbi.1998.1051
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Current information on the structure and function of motile tubular vacuoles in Pisolithus tinctorius and other fungi is reviewed, The use of fluorochromes to label the vacuole lumen is evaluated and observations on the structure and motility of vacuoles in P. tinctorius are differentiated from possible artifacts. The styryl dyes FM4-64 and MDY-64, used in yeast to demonstrate endocytosis, show little or no labeling of internal membranes in undamaged P, tinctorius cells. This agrees with our data showing that other probes for endocytosis such as Lucifer yellow CH are not taken up by hyphal tip cells. Overall, the observations do not support endocytosis in hyphal tips. It has been suggested that tubular vacuole systems carry out longitudinal transport, and evidence in favor of this hypothesis is evaluated. New data are presented to show that many of the large vacuoles in subapical cells are attached to the plasma membrane and are relatively immobile, while video sequences show movement of fluorochrome in pulses along a series of several large vacuoles, all interconnected via tubules, Tubular vacuoles from thick sections of hyphae processed under anhydrous conditions are shown by X-ray microanalysis to contain relatively high levels of P and K, as seen previously in the larger vacuoles. These results provide further evidence for a role of the tubular vacuoles in longitudinal transport of P. (C) 1998 Academic Press.
引用
收藏
页码:86 / 100
页数:15
相关论文
共 47 条
  • [1] Vacuolar reticulum in oomycete hyphal tips:: An additional component of the Ca2+ regulatory system?
    Allaway, WG
    Ashford, AE
    Heath, IB
    Hardham, AR
    [J]. FUNGAL GENETICS AND BIOLOGY, 1997, 22 (03) : 209 - 220
  • [2] Ashford AE, 1998, ADV BOT RES, V28, P119
  • [3] DEMONSTRATION OF A SHORT-CHAIN POLYPHOSPHATE IN PISOLITHUS-TINCTORIUS AND THE IMPLICATIONS FOR PHOSPHORUS TRANSPORT
    ASHFORD, AE
    RYDE, S
    BARROW, KD
    [J]. NEW PHYTOLOGIST, 1994, 126 (02) : 239 - 247
  • [4] ASHFORD AE, 1995, CURRENT TOPICS PLANT, P135
  • [5] INVITRO DEVELOPMENT OF PENETRATION STRUCTURES IN THE RICE BLAST FUNGUS MAGNAPORTHE-GRISEA
    BOURETT, TM
    HOWARD, RJ
    [J]. CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1990, 68 (02): : 329 - 342
  • [6] POSTEMBEDMENT LABELING OF INTRACELLULAR CONCANAVALIN A-BINDING SITES IN FREEZE-SUBSTITUTED FUNGAL CELLS
    BOURETT, TM
    PICOLLELLI, MA
    HOWARD, RJ
    [J]. EXPERIMENTAL MYCOLOGY, 1993, 17 (03): : 223 - 235
  • [7] Interactions between Pisolithus tinctorius and its hosts: a review of current knowledge
    Cairney, JWG
    Chambers, SM
    [J]. MYCORRHIZA, 1997, 7 (03) : 117 - 131
  • [8] INFLUENCE OF INTRACELLULAR PHOSPHORUS CONCENTRATION ON PHOSPHATE ABSORPTION BY THE ECTOMYCORRHIZAL BASIDIOMYCETE PISOLITHUS-TINCTORIUS
    CAIRNEY, JWG
    SMITH, SE
    [J]. MYCOLOGICAL RESEARCH, 1992, 96 : 673 - 676
  • [9] Uptake and compartmentalisation of fluorescent probes by Pisolithus tinctorius hyphae: evidence for an anion transport mechanism at the tonoplast but not for fluid-phase endocytosis
    Cole, L
    Hyde, GJ
    Ashford, AE
    [J]. PROTOPLASMA, 1997, 199 (1-2) : 18 - 29
  • [10] TUBULOVESICULAR PROCESSES EMERGE FROM TRANS-GOLGI CISTERNAE, EXTEND ALONG MICROTUBULES, AND INTERLINK ADJACENT TRANS-GOLGI ELEMENTS INTO A RETICULUM
    COOPER, MS
    CORNELLBELL, AH
    CHERNJAVSKY, A
    DANI, JW
    SMITH, SJ
    [J]. CELL, 1990, 61 (01) : 135 - 145