Immunochemical assay for satratoxin G and other macrocyclic trichothecenes associated with indoor air contamination by Stachybotrys chartarum

被引：22

作者：

Chung, YJ

Jarvis, BB

Tak, H

Pestka, JJ

机构：

[1] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA

[2] Michigan State Univ, Inst Environm Toxicol, E Lansing, MI 48824 USA

[3] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA

[4] Michigan State Univ, Dept Microbiol & Mol Genet, Inst Environm Toxicol, E Lansing, MI 48824 USA

来源：

TOXICOLOGY MECHANISMS AND METHODS | 2003年 / 13卷 / 04期

基金：

美国国家卫生研究院;

关键词：

ELISA; mycotoxin; satratoxin; Stachybotrys chartarum;

D O I：

10.1080/713857196

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Although satratoxin G (SG) is the primary macrocyclic trichothecene produced by Stachybotrys chartarum, a fungus frequently associated with outbreaks of indoor air illness, little is known about the actual quantities of this mycotoxin in suspect or normal building environments. Using antibodies produced, against an SG hemisuccinate-bovine serum albumin conjugate, we devised an enzyme-linked immunosorbent assay that enabled the detection of as little as 100 pg/mL of SG and that could also detect other members of the satratoxin family. Detection by this assay of satratoxins in methanolic extracts of S. chartarum in rice cultures was comparable to that of high-performance liquid chromatography, indicating that this assay will be useful in detecting SG and other satratoxins in environmental samples.

引用

页码：247 / 252

页数：6

共 32 条

[1] MACROCYCLIC TRICHOTHECENE TOXINS PRODUCED BY STACHYBOTRYS-ATRA STRAINS ISOLATED IN MIDDLE EUROPE [J].