Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of YopM, an essential virulence factor extruded by the plague bacterium Yersinia pestis

被引：13

作者：

Evdokimov, AG

Anderson, DE

Routzahn, KM

Waugh, DS

机构：

[1] NCI, Prot Engn Sect, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA

[2] NCI, Struct Biol Core Facil, Program Struct Biol, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2000年 / 56卷

关键词：

D O I：

10.1107/S0907444900013378

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant form of Yersinia pestis YopM with a C-terminal polyhistidine affinity tag has been overproduced in Escherichia coli, purified to homogeneity and crystallized using the hanging-drop vapor-diffusion technique. Several different crystal forms were obtained. The most suitable crystals for X-ray diffraction belonged to space groups P4(2)22 (unit-cell parameters a = 109.36, b = 109.36, c = 101.50 Angstrom) and C222(1) (unit-cell parameters a = 71.73, b = 121.85, c = 189.79 Angstrom). With a synchrotron-radiation source, these crystals diffracted to 2.4 and 1.9 Angstrom resolution, respectively.

引用

页码：1676 / 1679

页数：4

共 22 条

[1] Identification of an amino-terminal substrate-binding domain in the Yersinia tyrosine phosphatase that is required for efficient recognition of focal adhesion targets
Black, DS
Montagna, LG
Zitsmann, S
Bliska, JB
[J]. MOLECULAR MICROBIOLOGY, 1998, 29 (05) : 1263 - 1274
[2] The virulence plasmid of Yersinia, an antihost genome
Cornelis, GR
Boland, A
Boyd, AP
Geuijen, C
Iriarte, M
Neyt, C
Sory, MP
Stainier, I
[J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (04) : 1315 - +
[3] The Yersinia Yop virulon: A bacterial system for subverting eukaryotic cells
Cornelis, GR
WolfWatz, H
[J]. MOLECULAR MICROBIOLOGY, 1997, 23 (05) : 861 - 867
[4] Common themes in microbial pathogenicity revisited
Finlay, BB
Falkow, S
[J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1997, 61 (02) : 136 - +
[5] Type III secretion machines:: Bacterial devices for protein delivery into host cells
Galán, JE
Collmer, A
[J]. SCIENCE, 1999, 284 (5418) : 1322 - 1328
[6] A SECRETED PROTEIN-KINASE OF YERSINIA-PSEUDOTUBERCULOSIS IS AN INDISPENSABLE VIRULENCE DETERMINANT
GALYOV, EE
HAKANSSON, S
FORSBERG, A
WOLFWATZ, H
[J]. NATURE, 1993, 361 (6414) : 730 - 732
[7] YopT, a new Yersinia Yop effector protein, affects the cytoskeleton of host cells
Iriarte, M
Cornelis, GR
[J]. MOLECULAR MICROBIOLOGY, 1998, 29 (03) : 915 - 929
[8] Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused
Kapust, RB
Waugh, DS
[J]. PROTEIN SCIENCE, 1999, 8 (08) : 1668 - 1674
[9] PROTEINS WITH LEUCINE-RICH REPEATS
KOBE, B
DEISENHOFER, J
[J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 1995, 5 (03) : 409 - 416
[10] IDENTIFICATION AND LOCATION OF L-GLYCERATE, AN UNUSUAL ACYL SUBSTITUENT IN GELLAN GUM
KUO, MS
MORT, AJ
DELL, A
[J]. CARBOHYDRATE RESEARCH, 1986, 156 : 173 - 187

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