Pleurotaenium trabecula, a desmid of wetland biofilms:: The extracellular matrix and adhesion mechanisms

被引:28
作者
Domozych, David S. [1 ]
Elliott, Leah
Kiemle, Sarah N.
Gretz, Michael R.
机构
[1] Skidmore Coll, Dept Biol, Saratoga Springs, NY 12866 USA
[2] Skidmore Coll, Skidmore Imaging Ctr, Saratoga Springs, NY 12866 USA
[3] Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49911 USA
[4] Michigan Technol Univ, Biotechnol Res Ctr, Houghton, MI 49911 USA
关键词
adhesion; biofilm; desmid; EPS; Pleurotaenium;
D O I
10.1111/j.1529-8817.2007.00389.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Pleurotaenium trabecula (Ehren.) Nageli is a placoderm desmid that commonly occurs in wetland biofilms of the southeastern Adirondacks (NY, USA). It often displays a distinctive habit whereby the cell remains attached to the substrate via the polar end of one semicell, while the remainder of the cell is suspended in the water column. In this study, we examined the extracellular matrix (ECM) of this alga to elucidate its adhesion mechanisms and postadhesion behavior. The ECM consists of the following: (i) an extracellular polymeric substance (EPS), which includes polyanionic and sulfated polysaccharides; (ii) a thin pectin-containing primary cell wall, which is quickly sloughed off after postcytokinetic semicell expansion; and (iii) a thick secondary cell wall that is perforated with a distinct pore complex. Each pore of this complex possesses an external network of densely aggregated fibrils. Selective solubilization and immunolabeling studies suggest that these fibrillar aggregates or "adhesion centers" (i.e., ACs) contain arabinogalactan protein and are involved in initial adhesion of the cell to a substrate. We propose that postinitial adhesion behavior entails localized secretion of EPS derived from a large pool of EPS-containing vesicles situated in the peripheral cytoplasm. As the EPS absorbs water, hygroscopic pressure breaks the connections between the ACs on the cell wall and substrate and allows a portion of a cell to lift up into the water column.
引用
收藏
页码:1022 / 1038
页数:17
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