A virus-based nanoblock with tunable electrostatic properties

被引：67

作者：

Chatterji, A

Ochoa, WF

Ueno, T

Lin, TW

Johnson, JE

机构：

[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA USA

[2] Scripps Res Inst, Ctr Integrat & Mol Biosci, La Jolla, CA USA

来源：

NANO LETTERS | 2005年 / 5卷 / 04期

关键词：

D O I：

10.1021/nl048007s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Five different "HIS tag" mutants of cowpea mosaic virus were made by genetically introducing six contiguous histidine residues at various locations on the virus capsid. The mutant particles showed differential affinity for binding nickel, and their electrostatic properties could be controlled as a function of the protonation state of the exposed histidine sequence. The specific addressability of the HIS tag was corroborated by the selective modification of the histidine sequence with nanogold cross-linked to the Ni-NTA moiety.

引用

收藏

页码：597 / 602

页数：6

相关论文

共 50 条

[1] Constrained synthesis of cobalt oxide nanomaterials in the 12-subunit protein cage from Listeria innocua [J].

Allen, M ;

Willits, D ;

Young, M ;

Douglas, T .

INORGANIC CHEMISTRY, 2003, 42 (20) :6300-6305

[2]

Allen M, 2002, ADV MATER, V14, P1562, DOI 10.1002/1521-4095(20021104)14:21<1562::AID-ADMA1562>3.0.CO

[3]

2-D

[4] A model-based approach for determining orientations of biological macromolecules imaged by cryoelectron microscopy [J].

Baker, TS ;

Cheng, RH .

JOURNAL OF STRUCTURAL BIOLOGY, 1996, 116 (01) :120-130

[5] Metal-recognition by repeating polypeptides [J].

Brown, S .

NATURE BIOTECHNOLOGY, 1997, 15 (03) :269-272

[6] Chemical conjugation of heterologous proteins on the surface of cowpea mosaic virus [J].

Chatterji, A ;

Ochoa, W ;

Shamieh, L ;

Salakian, SP ;

Wong, SM ;

Clinton, G ;

Ghosh, P ;

Lin, TW ;

Johnson, JE .

BIOCONJUGATE CHEMISTRY, 2004, 15 (04) :807-813

[7] New addresses on an addressable virus nanoblock: Uniquely reactive lys residues on cowpea mosaic virus [J].

Chatterji, A ;

Ochoa, WF ;

Paine, M ;

Ratna, BR ;

Johnson, JE ;

Lin, TW .

CHEMISTRY & BIOLOGY, 2004, 11 (06) :855-863

[8] Cowpea mosaic virus: From the presentation of antigenic peptides to the display of active biomaterials [J].

Chatterji, A ;

Burns, LL ;

Taylor, SS ;

Lomonossoff, GP ;

Johnson, JE ;

Lin, T ;

Porta, C .

INTERVIROLOGY, 2002, 45 (4-6) :362-370

[9] CAULIFLOWER MOSAIC-VIRUS 35S PROMOTER-CONTROLLED DNA COPIES OF COWPEA MOSAIC-VIRUS RNAS ARE INFECTIOUS ON PLANTS [J].

DESSENS, JT ;

LOMONOSSOFF, GP .

JOURNAL OF GENERAL VIROLOGY, 1993, 74 :889-892

[10] Doughnut-shaped peptide nano-assemblies and their applications as nanoreactors [J].

Djalali, R ;

Samson, J ;

Matsui, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (25) :7935-7939

← 1 2 3 4 5 →