Excision of a selectable marker in transgenic rice (Oryza sativa L.) using a chemically regulated Cre/loxP system

被引：104

作者：

Sreekala, C ^{[1
]}

Wu, L ^{[1
]}

Gu, K ^{[1
]}

Wang, D ^{[1
]}

Tian, D ^{[1
]}

Yin, Z ^{[1
]}

机构：

[1] Natl Univ Singapore, Lab Mol Plant Pathol, Temasek Life Sci Lab, Singapore 117604, Singapore

来源：

PLANT CELL REPORTS | 2005年 / 24卷 / 02期

关键词：

marker-free transformation; rice; Cre; loxP; DNA recombination;

D O I：

10.1007/s00299-004-0909-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Removal of a selectable marker gene from genetically modified (GM) crops alleviates the risk of its release into the environment and hastens the public acceptance of GM crops. Here we report the production of marker-free transgenic rice by using a chemically regulated, Cre/loxP-mediated site-specific DNA recombination in a single transformation. Among 86 independent transgenic lines, ten were found to be marker-free in the T-0 generation and an additional 17 lines segregated marker-free transgenic plants in the T-1 generation. Molecular and genetic analyses indicated that the DNA recombination and excision in transgenic rice were precise and the marker-free recombinant T-DNA was stable and heritable.

引用

页码：86 / 94

页数：9

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