Na+,K+-ATPase was found to be the membrane component responsible for the hydrophobic behavior of the brain membrane tubulin

被引：20

作者：

Alonso, ADC ^{[1
]}

Nuñez-Fernandez, M ^{[1
]}

Beltramo, DM ^{[1
]}

Casale, CH ^{[1
]}

Barra, HS ^{[1
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Quim Biol, CONICET,CIQUIBIC, RA-5000 Cordoba, Argentina

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 253卷 / 03期

关键词：

D O I：

10.1006/bbrc.1998.9859

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously described that the tubulin isolated from brain membranes as a hydrophobic compound by partitioning into Triton X-114, The hydrophobic behavior of this tubulin is due to its interaction with membrane protein(s) and the interaction occurs principally with the acetylated tubulin isotype, In the present work we identified the membrane protein that interacts with tubulin as the Na+,K+-ATPase alpha subunit by amino acid sequencing. Using purified brain Na+,K+-ATPase we were able to isolate part of the total hydrophilic tubulin as a hydrophobic compound which contains a high proportion of the acetylated tubulin isotype. (C) 1998 Academic Press.

引用

页码：824 / 827

页数：4

共 21 条

[1] SYNAPTIC PLASMA-MEMBRANE TUBULIN MAY BE AN INTEGRAL CONSTITUENT [J].