Docosahexaenoic acid affects cell signaling by altering lipid rafts

被引:197
作者
Stillwell, W
Shaikh, SR
Zerouga, M
Siddiqui, R
Wassall, SR
机构
[1] Indiana Univ Purdue Univ, Dept Biol, Indianapolis, IN 46202 USA
[2] Clarian Hlth Partners, Methodist Res Inst, Cellular Biochem Lab, Indianapolis, IN 46206 USA
[3] Indiana Univ Purdue Univ, Dept Phys, Indianapolis, IN 46202 USA
来源
REPRODUCTION NUTRITION DEVELOPMENT | 2005年 / 45卷 / 05期
关键词
apoptosis; docosahexaenoic acid; lipid rafts; membranes; phospholipids;
D O I
10.1051/rnd:2005046
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
With 22 carbons and 6 double bonds docosahexaenoic acid (DHA) is the longest and most unsaturated fatty acid commonly found in membranes. It represents the extreme example of a class of important human health promoting agents known as omega-3 fatty acids. DHA is particularly abundant in retinal and brain tissue, often comprising about 50% of the membrane's total acyl chains. Inadequate amounts of DHA have been linked to a wide variety of abnormalities ranging from visual acuity and learning irregularities to depression and suicide. The molecular mode of action of DHA, while not yet understood, has been the focus of our research. Here we briefly summarize how DHA affects membrane physical properties with an emphasis on membrane signaling domains known as rafts. We report the uptake of DHA into brain phosphatidylethanolamines and the subsequent exclusion of cholesterol from the DHA-rich membranes. We also demonstrate that DHA-induced apoptosis in MDA-MB-231 breast cancer cells is associated with externalization of phosphatidylserine and membrane disruption ("blebbing"). We conclude with a proposal of how DHA incorporation into membranes may control cell biochemistry and physiology.
引用
收藏
页码:559 / 579
页数:21
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