Identification of the major tamoxifen-DNA adducts in rat liver by mass spectroscopy

被引：46

作者：

Rajaniemi, H ^{[1
]}

Rasanen, I

Koivisto, P

Peltonen, K

Hemminki, K

机构：

[1] Karolinska Inst, Novum, Dept Biosci, S-14157 Huddinge, Sweden

[2] Univ Helsinki, Dept Forens Med, FIN-00014 Helsinki, Finland

[3] Inst Occupat Hlth, FIN-00250 Helsinki, Finland

来源：

CARCINOGENESIS | 1999年 / 20卷 / 02期

关键词：

D O I：

10.1093/carcin/20.2.305

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We present here the first mass spectroscopic (MS) identification of the main tamoxifen-induced DNA adducts in rat liver. The two main adducts were isolated by highperformance liquid chromatography (HPLC) and identified by MS, MS-MS and ultraviolet spectroscopy. Adduct 1 was the N-desmethyltamoxifen-deoxyguanosine adduct in which the a-position of the metabolite N-desmethyltamoxifen is linked covalently to the amino group of deoxyguanosine, Adduct 2 was confirmed to be the trans isomer of a(N-2-deoxyguanosinyl)tamoxifen, as previously suggested by co-chromatography.

引用

页码：305 / 309

页数：5

共 25 条

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