Oxygen consumption of single bovine embryos probed by scanning electrochemical microscopy

被引:158
作者
Shiku, H
Shiraishi, T
Ohya, H
Matsue, T
Abe, H
Hoshi, H
Kobayashi, M
机构
[1] Japan Sci & Technol Corp, Reg Joint Res Project Yamagata Prefecture, Yamagata 9902473, Japan
[2] Inst Life Support Technol, Yamagata 9902473, Japan
[3] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Sendai, Miyagi 9808579, Japan
[4] Res Inst Funct Peptides, Yamagata 9900823, Japan
[5] Yamagata Agr Res & Training Ctr, Shinjo 9960041, Japan
关键词
D O I
10.1021/ac010339j
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Oxygen consumption of individual bovine embryos was noninvasively quantified by scanning electrochemical microscopy (SECM). A probe microelectrode was used to scan near a single embryo surface in a culture medium to monitor the oxygen reduction current at 37 degreesC, under a water-saturated atmosphere of 5% CO2 and 95% air. The oxygen concentration profiles near the embryos were in good agreement with the theoretical spherical diffusion. When an embryo reached the stage of a morula with a 74-mum radius on day 6 after in Nitro fertilization, the oxygen concentration difference (DeltaC) between the bulk solution and the morula surface was 6.90 +/- 1.35 muM. The oxygen consumption rate (F) of the single morula was estimated to be (1.40 +/- 0.27) x 10(-14) Mol s(-1). After the SECM measurement, the embryo was continuously cultured for another 2 days and grew to the stage of a blastocyst with a 100-mum radius. For the blastocyst, the DeltaC values for the inner cell mass side and the trophoblast side were 16.40 +/- 1.83 and 9.14 +/- 1.68 muM, respectively. The oxygen consumption rate of the blastocyst was found to be in the range of (2.50 +/- 0.46) x 10(-14) Mol S-1 < F < (4.49 +/- 0.50) x 10(-14) Mol s(-1). We have carried out SECM measurements for 19 embryos, and the results were compared in detail with these from an optical microscopic observation. The DeltaC values for the morulae on day 6 after in vitro fertilization were strongly related to the morphological embryo quality. The morulae showing a larger DeltaC value developed into blastocysts of a larger size, and the DeltaC value after the subsequent 2 days of cultivation was found to be increased.
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页码:3751 / 3758
页数:8
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