NMR observation of Tau in Xenopus oocytes

被引：81

作者：

Bodart, Jean-Francois ^{[2
,4
]}

Wieruszeski, Jean-Michel ^{[1
]}

Amniai, Laziza ^{[1
]}

Leroy, Arnaud ^{[1
,3
]}

Landrieu, Isabelle ^{[1
]}

Rousseau-Lescuyer, Arlette ^{[2
]}

Vilain, Jean-Pierre ^{[2
]}

Lippens, Guy ^{[1
]}

机构：

[1] Univ Sci & Tech Lille Flandres Artois, CNRS, UMR8576, Glycobiol Struct & Fonctionnelle, F-59655 Villeneuve Dascq, France

[2] Univ Sci & Tech Lille Flandres Artois, Lab Regulat Signaux Div, F-59655 Villeneuve Dascq, France

[3] Fac Pharm Chatenay Malabry Paris XI, Chatenay Malabry, France

[4] Univ Sci & Tech Lille Flandres Artois, Inst Rech Interdisciplinaire, CNRS, FRE 2963, F-59655 Villeneuve Dascq, France

来源：

JOURNAL OF MAGNETIC RESONANCE | 2008年 / 192卷 / 02期

关键词：

NMR; in-cell; Xenopus oocytes; ficoli; cell survival; Tau; phosphorylation;

D O I：

10.1016/j.jmr.2008.03.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The observation by NMR spectroscopy of microinjected N-15-labelled proteins into Xenopus laevis oocytes might open the way to link structural and cellular biology. We show here that embedding the oocytes into a 20% Ficoll solution maintains their structural integrity over extended periods of time, allowing for the detection of nearly physiological protein concentrations. We use these novel conditions to study the neuronal Tau protein inside the oocytes. Spectral reproducibility and careful comparison of the spectra of Tau before and after cell homogenization is presented. When injecting Tau protein into immature oocytes, we show that both its microtubule association and different phosphorylation events can be detected. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：252 / 257

页数：6

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