Rapid microwave-assisted dansylation of biogenic amines -: Analysis by high-performance liquid chromatography

被引：26

作者：

Linares, RM ^{[1
]}

Ayala, JH ^{[1
]}

Afonso, AM ^{[1
]}

Díaz, VG ^{[1
]}

机构：

[1] Univ La Laguna, Dept Analyt Chem Nutr & Food Sci, E-38201 La Laguna, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 808卷 / 1-2期

关键词：

microwave-assisted dansylation; derivatization; LC; biogenic amines; putrescine; cadaverine; spermidine; spermine;

D O I：

10.1016/S0021-9673(98)00105-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast microwave-assisted dansylation procedure has been developed for the derivatization of biogenic amines prior to HPLC determination. Putrescine, cadaverine, spermidine and spermine are quantitatively dansylated in 5 min using a radiation power of 252 W and a maximum pressure of 3.4 bar inside the reactor. As compared to classical overnight dansylation, the microwave-assisted procedure is 150-times faster. Data concerning repeatability and recovery of method are reported. The method was successfully applied to the determination of the above amines in plant tissues. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：87 / 93

页数：7

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