Migration behavior of dyestuff-containing liposomes in capillary electrophoresis with chemiluminescence detection

Various types of dyestuff-containing liposomes were prepared from eosin Y or rhodaminine B, dipalmitoylphosphatidylcholine or dipalmitoylphosphatidylcholine-dipalmitoylphosphatidylserine mixtures, and buffer solutions (trapped solutions) differing in chemical species, buffer concentrations, and pH. The dyestuff-containing liposomes were subjected to capillary electrophoresis with chemiluminescence detection, and the effects of the constituents on liposome properties were examined from the obtained electropherogram. Two peaks were typically recorded on the electropherograms; one was due to dyestuff entrapped in the liposomes and another was due to free dyestuff in the bulk solution. The changes of retention times and ratios of the two peaks easily and rapidly offered useful information as to permeability and surface charge of the liposome membranes. (C) 1998 Elsevier Science B.V. All rights reserved.