Acetylcholine enzyme sensor for determining methamidophos insecticide -: Evaluation of some genetically modified acetylcholinesterases from Drosophila melanogaster

被引：48

作者：

Nunes, GS

Montesinos, T

Marques, PBO

Fournier, D

Marty, JL

机构：

[1] Univ Perpignan, Ctr Phytopharm, UMRA CNRS 5054, F-66860 Perpignan, France

[2] Univ Fed Maranhao, Ctr Tecnol, Dept Tecnol Quim, BR-65080040 Sao Luis, MA, Brazil

[3] Univ Toulouse 3, Lab Synth & Physicochim Mol Interet Biol, ESA 568, F-31062 Toulouse, France

来源：

ANALYTICA CHIMICA ACTA | 2001年 / 434卷 / 01期

关键词：

screen-printed electrodes; methamidophos; recombinant acetylcholinesterases; inhibition constant (k(i));

D O I：

10.1016/S0003-2670(01)00795-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive screen-printed amperometric sensor suitable for rapid determination of the concentration of the insecticide methamidophos was developed. It was based on the principle of inhibition of acetylcholinesterase (AChE) activity. The first part of the study was focused on the screening of several genetically modified AChEs in order to select the most sensitive enzyme towards the methamidophos. Values for the bimolecular constant k(i) were also determined. In a second part of the study, we compared the lowest detectable methamidophos concentrations using different immobilised AChEs. The lowest detectable concentration in a standard solution was 1.4ppb for the AChE(Dros)-B03 mutant compared to 4.8 ppb for the wild type AChE and 53 ppb for the electric eel source. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 8

页数：8

共 25 条

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