P Bodies Inhibit Retrotransposition of Endogenous Intracisternal A Particles

被引：16

作者：

Lu, Chunye ^{[1
,2
]}

Contreras, Xavier ^{[1
,2
]}

Peterlin, B. Matija ^{[1
,2
,3
]}

机构：

[1] Univ Calif San Francisco, Rosalind Russell Med Res Ctr, Dept Med, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Rosalind Russell Med Res Ctr, Dept Microbiol & Immunol, San Francisco, CA 94143 USA

[3] Univ Helsinki, Dept Virol, Haartman Inst, Helsinki 00014, Finland

来源：

JOURNAL OF VIROLOGY | 2011年 / 85卷 / 13期

关键词：

CYTOPLASMIC PROCESSING BODIES; MESSENGER-RNA DECAY; MAMMALIAN STRESS GRANULES; HIV-1; REPLICATION; DECAPPING FACTORS; MOUSE-LIVER; GENE; TRANSLATION; EXPRESSION; MICRORNAS;

D O I：

10.1128/JVI.02517-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

mRNA-processing bodies (P bodies) are cytoplasmic foci that contain translationally repressed mRNA. Since they are important for the retrotransposition of Ty elements and brome mosaic virus in yeast cells, we assessed the role of P bodies in the movement of endogenous intracisternal A particles (IAPs) in mammalian cells. In contrast to the case for these other systems, their disruption via knockdown of RCK or eukaryotic initiation factor E transporter (eIF4E-T) increased IAP retrotransposition as well as levels of IAP transcripts, Gag proteins, and reverse transcription products. This increase was not mediated by impairing the microRNA pathway. Rather, the removal of P bodies shifted IAP mRNA from nonpolysomal to polysomal fractions. Although IAP mRNA localized to P bodies, Gag was targeted to the endoplasmic reticulum (ER), from which IAP buds. Thus, by sequestering IAP mRNA away from Gag, P bodies inhibit rather than promote IAP retrotransposition.

引用

页码：6244 / 6251

页数：8

共 45 条

[1] A role for eIF4E and eIF4E-transporter in targeting mRNPs to mammalian processing bodies [J].