A Commensal Gone Bad: Complete Genome Sequence of the Prototypical Enterotoxigenic Escherichia coli Strain H10407

被引：147

作者：

Crossman, Lisa C. ^{[2
]}

Chaudhuri, Roy R. ^{[3
]}

Beatson, Scott A. ^{[4
]}

Wells, Timothy J. ^{[1
]}

Desvaux, Mickael ^{[1
]}

Cunningham, Adam F. ^{[1
]}

Petty, Nicola K. ^{[2
]}

Mahon, Vivienne ^{[6
]}

Brinkley, Carl ^{[7
]}

Hobman, Jon L. ^{[9
]}

Savarino, Stephen J. ^{[8
]}

Turner, Susan M. ^{[1
]}

Pallen, Mark J. ^{[5
]}

Penn, Charles W. ^{[5
]}

Parkhill, Julian ^{[2
]}

Turner, A. Keith ^{[2
]}

Johnson, Timothy J. ^{[10
]}

Thomson, Nicholas R. ^{[2
]}

Smith, Stephen G. J. ^{[6
]}

Henderson, Ian R. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Immun & Infect, Birmingham B15 2TT, W Midlands, England

[2] Wellcome Trust Sanger Inst, Cambridge, England

[3] Univ Cambridge, Dept Vet Med, Cambridge, England

[4] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld, Australia

[5] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

[6] Trinity Coll Dublin, Sch Med, Dept Clin Microbiol, Dublin, Ireland

[7] Walter Reed Army Inst Res, Dept Enter Infect, Silver Spring, MD USA

[8] Enter Dis Dept, Silver Spring, MD USA

[9] Univ Nottingham, Sch Biosci, Loughborough, England

[10] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA

来源：

JOURNAL OF BACTERIOLOGY | 2010年 / 192卷 / 21期

基金：

英国生物技术与生命科学研究理事会;

关键词：

EPITHELIAL-CELL INVASION; HEAT-LABILE ENTEROTOXIN; MOLECULAR CHARACTERIZATION; PLASMID STABILITY; GENE-CLUSTER; PROTEIN; IDENTIFICATION; SECRETION; COLONIZATION; VIRULENCE;

D O I：

10.1128/JB.00710-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible for a significant portion of pediatric deaths. In this study, we determined the complete genomic sequence of E. coli H10407, a prototypical strain of enterotoxigenic E. coli, which reproducibly elicits diarrhea in human volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains, revealing that the chromosome is closely related to that of the nonpathogenic commensal strain E. coli HS and to those of the laboratory strains E. coli K-12 and C. Furthermore, these analyses demonstrated that there were no chromosomally encoded factors unique to any sequenced ETEC strains. Comparison of the E. coli H10407 plasmids with those from several ETEC strains revealed that the plasmids had a mosaic structure but that several loci were conserved among ETEC strains. This study provides a genetic context for the vast amount of experimental and epidemiological data that have been published.

引用

页码：5822 / 5831

页数：10

共 66 条

[1]

SEPA, THE MAJOR EXTRACELLULAR PROTEIN OF SHIGELLA-FLEXNERI - AUTONOMOUS SECRETION AND INVOLVEMENT IN TISSUE INVASION [J].

BENJELLOUNTOUIMI, Z ;

SANSONETTI, PJ ;

PARSOT, C .

MOLECULAR MICROBIOLOGY, 1995, 17 (01) :123-135

[2]

MOLECULAR-GENETICS AND TRANSPORT ANALYSIS OF THE COPPER-RESISTANCE DETERMINANT (PCO) FROM ESCHERICHIA-COLI PLASMID PRJ1004 [J].

BROWN, NL ;

BARRETT, SR ;

CAMAKARIS, J ;

LEE, BTO ;

ROUCH, DA .

MOLECULAR MICROBIOLOGY, 1995, 17 (06) :1153-1166

[3]

Carbon nutrition of Escherichia coli in the mouse intestine [J].

Chang, DE ;

Smalley, DJ ;

Tucker, DL ;

Leatham, MP ;

Norris, WE ;

Stevenson, SJ ;

Anderson, AB ;

Grissom, JE ;

Laux, DC ;

Cohen, PS ;

Conway, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (19) :7427-7432

[4]

Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042 [J].

Chaudhuri, Roy R. ;

Sebaihia, Mohammed ;

Hobman, Jon L. ;

Webber, Mark A. ;

Leyton, Denisse L. ;

Goldberg, Martin D. ;

Cunningham, Adam F. ;

Scott-Tucker, Anthony ;

Ferguson, Paul R. ;

Thomas, Christopher M. ;

Frankel, Gad ;

Tang, Christoph M. ;

Dudley, Edward G. ;

Roberts, Ian S. ;

Rasko, David A. ;

Pallen, Mark J. ;

Parkhill, Julian ;

Nataro, James P. ;

Thomson, Nicholas R. ;

Henderson, Ian R. .

PLOS ONE, 2010, 5 (01)

[5]

Subtractive hybridization and optical mapping of the enterotoxigenic Escherichia coli H10407 chromosome:: isolation of unique sequences and demonstration of significant similarity to the chromosome of E coli K-12 [J].

Chen, Q ;

Savarino, SJ ;

Venkatesan, MM .

MICROBIOLOGY-SGM, 2006, 152 :1041-1054

[6]

Immune response, ciprofloxacin activity, and gender differences after human experimental challenge by two strains of enterotoxigenic Escherichia coli [J].

Coster, T. S. ;

Wolf, M. K. ;

Hall, E. R. ;

Cassels, F. J. ;

Taylor, D. N. ;

Liu, C. T. ;

Trespalacios, F. C. ;

DeLorimier, A. ;

Angleberger, D. R. ;

McQueen, C. E. .

INFECTION AND IMMUNITY, 2007, 75 (01) :252-259

[7]

CRAVIOTO A, 1980, THESIS U LONDON LOND

[8]

THE HEAT-STABLE TOXIN-I GENE FROM ESCHERICHIA-COLI 18D [J].

DALLAS, WS .

JOURNAL OF BACTERIOLOGY, 1990, 172 (09) :5490-5493

[9]

THE NUCLEOTIDE-SEQUENCE OF A REGULATORY GENE PRESENT ON A PLASMID IN AN ENTEROTOXIGENIC ESCHERICHIA-COLI STRAIN OF SEROTYPE O167-H5 [J].

DEHAAN, LAM ;

WILLSHAW, GA ;

VANDERZEIJST, BAM ;

GAASTRA, W .

FEMS MICROBIOLOGY LETTERS, 1991, 83 (03) :341-346

[10]

Improved microbial gene identification with GLIMMER [J].

Delcher, AL ;

Harmon, D ;

Kasif, S ;

White, O ;

Salzberg, SL .

NUCLEIC ACIDS RESEARCH, 1999, 27 (23) :4636-4641

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