Myocyte vascular endothelial growth factor is required for exercise-induced skeletal muscle angiogenesis

被引:99
作者
Olfert, I. Mark [1 ,2 ]
Howlett, Richard A. [2 ]
Wagner, Peter D. [2 ]
Breen, Ellen C. [2 ]
机构
[1] W Virginia Univ, Div Exercise Physiol, Sch Med, Ctr Cardiovasc & Resp Sci, Morgantown, WV 26506 USA
[2] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA
关键词
mouse; knockout; training; capillary; GENE-EXPRESSION; ENZYME-ACTIVITY; VEGF; CAPACITY; CAPILLARITY; TRANSCRIPTION; ADAPTATIONS; HIF-1-ALPHA; GRADIENTS; RESPONSES;
D O I
10.1152/ajpregu.00347.2010
中图分类号
Q4 [生理学];
学科分类号
071003 [生理学];
摘要
Olfert IM, Howlett RA, Wagner PD, Breen EC. Myocyte vascular endothelial growth factor is required for exercise-induced skeletal muscle angiogenesis. Am J Physiol Regul Integr Comp Physiol 299: R1059-R1067, 2010. First published August 4, 2010; doi:10.1152/ajpregu.00347.2010.-We have previously shown, using a Cre-LoxP strategy, that vascular endothelial growth factor (VEGF) is required for the development and maintenance of skeletal muscle capillarity in sedentary adult mice. To determine whether VEGF expression is required for skeletal muscle capillary adaptation to exercise training, gastrocnemius muscle capillarity was measured in myocyte-specific VEGF gene-deleted (mVEGF(-/-)) and wild-type (WT) littermate mice following 6 wk of treadmill running (1 h/day, 5 days/wk) at the same running speed. The effect of training on metabolic enzyme activity levels and whole body running performance was also evaluated in mVEGF(-/-) and WT mice. Posttraining capillary density was significantly increased by 59% (P < 0.05) in the deep muscle region of the gastrocnemius in WT mice but did not change in mVEGF(-/-) mice. Maximal running speed and time to exhaustion during submaximal running increased by 20 and 13% (P < 0.05), respectively, in WT mice after training but were unchanged in mVEGF(-/-) mice. Training led to increases in skeletal muscle citrate synthase (CS) and phosphofructokinase (PFK) activities in both WT and mVEGF(-/-) mice (P < 0.05), whereas beta-hydroxyacyl-CoA dehydrogenase (beta-HAD) activity was increased only in WT mice. These data demonstrate that skeletal muscle capillary adaptation to physical training does not occur in the absence of myocyte-expressed VEGF. However, skeletal muscle metabolic adaptation to exercise training takes place independent of myocyte VEGF expression.
引用
收藏
页码:R1059 / R1067
页数:9
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